Document Detail

Comparison of the effect of fish oil and corn oil on chemical-induced hepatic enzyme-altered foci in rats.
MedLine Citation:
PMID:  10995328     Owner:  NLM     Status:  MEDLINE    
The effects of fish oil and corn oil diets on diethylnitrosamine initiation/phenobarbital promotion of hepatic enzyme-altered foci in female Sprague-Dawley rats were investigated. Groups of 12 rats were initiated with diethylnitrosamine (15 mg/kg) at 24 h of age. After weaning, they received diets containing either 13.5% fish oil plus 1. 5% corn oil or 15% corn oil for 24 weeks. Rats fed fish oil had significantly greater liver weight, relative liver weight, spleen weight, and relative spleen weight than rats fed corn oil (p < 0.05). Hepatic phospholipid fatty-acid profile was significantly affected by the type of dietary lipid. The rats fed fish oil had significantly greater hepatic phospholipid 20:5 and 22:6 than rats fed corn oil; in contrast, the rats fed corn oil had significantly greater hepatic phospholipid 18:2 and 20:4 than rats fed fish oil (p < 0.05). Rats fed fish oil had significantly lower hepatic vitamin E and PGE(2) content but significantly greater hepatic lipid peroxidation than rats fed corn oil (p < 0.05). The hepatic levels of antioxidant enzymes (GSH reductase and GST) were significantly greater in rats fed fish oil than in rats fed corn oil (p < 0.05). Except for PGST-positive foci (foci area/tissue area), all the other foci parameters (GGT-positive foci area/tissue area, GGT-positive foci no./cm(2), GGT-positive foci no./cm(3), PGST-positive foci no. /cm(2), and PGST-positive foci no./cm(3)) measured in the fish oil group were 10-30% of those in the corn oil group (p < 0.05). Analyses of Pearson correlation coefficient revealed a positive correlation between hepatic GGT- or PGST-positive foci number (no. /cm(2)) and PGE(2) content (r = 0.66, P = 0.01; r = 0.56, P = 0.02, respectively) but a negative correlation between GGT- and PGST-positive foci (no./cm(2)) and lipid peroxidation (r = -0.8, P = 0.0006; r = -0.58, P = 0.01, respectively), GSH/(GSH + GSSG) ratio (r = -0.61, P = 0.05; r = -0.4, P = 0.14, respectively), GSH reductase (r = -0.75, P = 0.002; r = -0.53, P = 0.02, respectively), and GST activities (r = -0.65, P = 0.01; r = -0.44, P = 0.07, respectively). Similar correlation between foci number (no./cm(3)) and PGE(2), lipid peroxidation, GSH/(GSH + GSSG) ratio, GSH reductase, and GST activities were obtained. The results of this study show that dietary fish oil significantly inhibited hepatic enzyme-altered foci formation compared with corn oil in rats. These results suggest that the possible mechanisms involved in this process are the stimulation of hepatic detoxification system, changes in membrane composition, inhibition of PGE(2) synthesis, the enhancement of GSH-related antioxidant capacity, and the enhancement of lipid peroxidation by fish oil.
Y J Ko; C K Lii; C C Ou; J Y Liu; W L Lin; H W Chen
Related Documents :
2844188 - Vitamin e influences the effects of fish oil on fatty acids and eicosanoid production i...
25386398 - Lipid-lowering activity of cow urine ark in guinea pigs fed with a high cholesterol diet.
1569378 - Dependence on dietary cholesterol for n-3 polyunsaturated fatty acid-induced changes in...
16675608 - Achene structure, development and lipid accumulation in sunflower cultivars differing i...
9734848 - Effects of extruded corn or grain sorghum on intake, digestibility, weight gain, and ca...
845688 - Effects of cellulose added to diets of low and high fiber content upon the metabolism o...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  48     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2000-11-13     Completed Date:  2000-11-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4144-50     Citation Subset:  IM    
Department of Nutrition, Institute of Biochemistry, Chung Shan Medical College, Taichung, Taiwan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Corn Oil / pharmacology*
Fish Oils / pharmacology*
Glutathione Transferase / metabolism
Liver / drug effects*,  enzymology
Liver Neoplasms, Experimental / enzymology,  pathology,  prevention & control*
Precancerous Conditions / enzymology,  pathology
Rats, Sprague-Dawley
gamma-Glutamyltransferase / metabolism
Reg. No./Substance:
0/Fish Oils; 8001-30-7/Corn Oil; EC; EC Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Lipid hydroperoxide determination in dark chicken meat through a ferrous oxidation-xylenol orange me...
Next Document:  Identification of biliary metabolites of (-)-epigallocatechin gallate in rats.