| Comparison of early passage, senescent and hTERT immortalized endothelial cells. | |
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MedLine Citation:
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PMID: 15964568 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The need for standardized experimental conditions to gain relevant and reproducible results has increased the demand for well characterized continuously growing cell lines that exhibit the characteristics of their normal counterparts. Immortalization of normal human cells by ectopic expression of the catalytic subunit of human telomerase (hTERT) has shown to result in highly differentiated cell lines. However, the influence of the increased telomerase activity on the protein expression profile was not investigated so far. Therefore, we have immortalized human umbilical vein endothelial cells (HUVECs) by hTERT overexpression and compared them to their normal early passage and senescent counterparts. This study, including a proteomic approach, shows that ectopic hTERT expression leads to a stable growing cell line. Although these cells are highly differentiated, the protein expression profile of the cell line is different to that of normal early passage and senescent cells. |
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Authors:
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Martina Wei-Fen Chang; Johannes Grillari; Corina Mayrhofer; Klaus Fortschegger; Günter Allmaier; Gorji Marzban; Hermann Katinger; Regina Voglauer |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental cell research Volume: 309 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 2005 Sep |
Date Detail:
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Created Date: 2005-08-26 Completed Date: 2005-12-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: United States |
Other Details:
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Languages: eng Pagination: 121-36 Citation Subset: IM |
Affiliation:
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Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A-1190 Vienna, Austria. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Aging
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physiology* Cell Differentiation / physiology Cell Division / physiology Cell Line Cell Line, Transformed / physiology* DNA-Binding Proteins* / genetics Electrophoresis, Gel, Two-Dimensional Endothelial Cells / cytology*, pathology, physiology* Gene Expression Regulation* Humans Polymerase Chain Reaction Proteomics Telomerase* / genetics Transfection |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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