| Comparison of the duplex-destabilizing effects of nucleobase-caged oligonucleotides. | |
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MedLine Citation:
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PMID: 20953770 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Nucleobase-caged oligonucleotide residues have photolabile "caging groups" that prevent the formation of Watson-Crick base pairs until the unmodified nucleobase is restored in a photolysis event. This principle can be used to put a growing variety of powerful nucleic acid-based applications under the precise spatiotemporal control using light as an addressing mechanism. Examples for applications include light control of transcription, RNAi, nucleic acid folding, primer extension, and restriction endonuclease as well as DNAzyme, aptamer, and antisense activity. However, a comparison of the duplex-destabilization properties of the various caged residues that have been used up to date and rules for achieving a maximal duplex destabilization with a minimum amount of modified residues are still missing. We present both a comparison of the duplex-destabilizing capabilities of various nucleobase-caged residues and address the question of influence on neighboring base pairs. |
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Authors:
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Alexandre Rodrigues-Correia; Martin B Koeppel; Florian Schäfer; K B Joshi; Timo Mack; Alexander Heckel |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-15 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 399 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-03 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 441-7 Citation Subset: IM |
Affiliation:
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Cluster of Excellence Macromolecular Complexes, Frankfurt Institute for Molecular Life Sciences, University of Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt am Main, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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