Document Detail

Comparison of direct swim-up, mini-Percoll, and Sephadex G10 separation procedures.
MedLine Citation:
PMID:  8166579     Owner:  NLM     Status:  MEDLINE    
A comparison was made of three sperm separation techniques--direct swim-up, mini-Percoll, and Sephadex G-10--on semen parameters, including count, % motility, forward progression, and hypoosmotic swelling (HOS) test scores. Overall, the best quality sperm (less debris, best % and quality motility, and best HOS scores) were noted with the direct swim-up procedure. Mini-Percoll resulted in the highest count, but the worst HOS score. Sephadex G-10 resulted in quality of semen specimen almost as good as the direct swim-up procedure, but did allow some noncellular debris and bacteria to filter through the column.
A Bollendorf; J H Check; D Katsoff; D Lurie
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Archives of andrology     Volume:  32     ISSN:  0148-5016     ISO Abbreviation:  Arch. Androl.     Publication Date:    1994 Mar-Apr
Date Detail:
Created Date:  1994-05-24     Completed Date:  1994-05-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7806755     Medline TA:  Arch Androl     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  157-62     Citation Subset:  IM    
University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden.
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MeSH Terms
Cell Separation / methods*
Centrifugation, Density Gradient
Chromatography, Gel
Sperm Count
Sperm Motility
Spermatozoa* / physiology

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