Document Detail


Comparison of different primers for rapid detection of Salmonella using the polymerase chain reaction.
MedLine Citation:
PMID:  10508555     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Salmonella is the leading cause of food-borne diarrhoeas in the US. In recent years polymerase chain reaction (PCR) has become the method of choice for rapid and sensitive detection of Salmonellae in contaminated foods. As a result, several different primer sets have been reported for use in PCR-based assay systems. In order to identify an optimal primer set from among the wide range of primers reported in the literature, we synthesized five different pairs and evaluated their relative performance in PCR under uniform assay conditions using a common panel of the target (Salmonella) and non-target (non- Salmonella) bacterial strains. Of the five sets of primers tested, the one designed on the basis of a 199 bp repeat sequence of S. weltevreden[Jitrapakdee et al. (1995) Molecular and Cellular Probes 9, 375-382] gave optimal results with most bacterial strains examined.
Authors:
C M Gooding; P V Choudary
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular probes     Volume:  13     ISSN:  0890-8508     ISO Abbreviation:  Mol. Cell. Probes     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-30     Completed Date:  1999-11-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8709751     Medline TA:  Mol Cell Probes     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  341-7     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Affiliation:
Antibody Engineering Laboratory and the Department of Entomology, University of California, Davis, CA, 95616-8584, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA Primers / standards*
DNA, Bacterial / analysis*
Polymerase Chain Reaction / instrumentation,  methods
Salmonella / genetics*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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