Document Detail


Comparison of different methods for an accurate assessment of cytotoxicity in the in vitro micronucleus test. I. Theoretical aspects.
MedLine Citation:
PMID:  18602494     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A decrease in the cytokinesis-block proliferation index (CBPI) or replication index (RI) is routinely used to determine cytotoxicity of a test compound and therefore the choice of its appropriate test concentration for the in vitro micronucleus (MN) test conducted in the presence of cytochalasin B. As a number of laboratories prefer to conduct the in vitro MN test in the absence of cytochalasin B, it is important that selected test concentrations, based on cytotoxicity, should be similar to what they would have been if cytochalasin B had been used, and should be relevant of a true cytotoxicity. By using models to analyse the dynamics of the cell cultures with and without cytochalasin B we have compared different methods for evaluation of cytotoxicity, and demonstrate that relative decrease in population doubling or relative increase in cell counts are the most appropriate measures of cytotoxicity to compare with reduction in CBPI or RI.
Authors:
E Lorge; M Hayashi; S Albertini; D Kirkland
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mutation research     Volume:  655     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:    2008 Aug-Sep
Date Detail:
Created Date:  2008-12-12     Completed Date:  2008-12-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1-3     Citation Subset:  IM    
Affiliation:
Servier Group, Drug Safety Assessment, BP 43255, 45403 Fleury-les-Aubrais, France. elisabeth.lorge@fr.netgrs.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Count
Cell Line, Tumor
Cell Proliferation
Cytochalasin B / metabolism
Mice
Micronucleus Tests / methods*,  standards
Models, Theoretical*
Chemical
Reg. No./Substance:
14930-96-2/Cytochalasin B

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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