Document Detail


Comparison of different cell lines and incubation times in the isolation by the shell vial culture of human metapneumovirus from pediatric respiratory samples.
MedLine Citation:
PMID:  17644404     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report a prospective study concerning the efficacy of LLC-MK2 (continuous monkey kidney cell), Hep-2, MDCK (Madin-Darby Canine Kidney), Vero and MRC-5 cell lines, by shell vial assay, and incubation time in the isolation of hMPV from pediatric respiratory samples. The overall sensitivity of the cell lines studied were: 100% for the LLC-MK2, 68.7% for the Hep-2, 28.1% for the Vero, 3.1% for the MDCK and 0% for the MRC-5. Only one strain (3.1%) showed growth in the four cell lines studied and 10 (31.2%) strains only grew in the LLC-MK2 cell line. The analysis of incubation times showed that only 14 strains (43.7%) were able to grow after 3 days of incubation, while all strains (100%) showed growth after 5 days. The use of shell vials with commercial LLC-MK2 cells could be a method for isolating hMPV from respiratory samples in the pediatric population.
Authors:
Jordi Reina; Francesc Ferres; Eva Alcoceba; Aina Mena; Enrique Ruiz de Gopegui; Joan Figuerola
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-07-17
Journal Detail:
Title:  Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology     Volume:  40     ISSN:  1386-6532     ISO Abbreviation:  J. Clin. Virol.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-31     Completed Date:  2007-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815671     Medline TA:  J Clin Virol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  46-9     Citation Subset:  IM    
Affiliation:
Virology Unit, Clinical Microbiology Service, University Hospital Son Dureta, Andrea Doria 55, 07014 Palma de Mallorca, Spain. jreina@hsd.es
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Child
Humans
Metapneumovirus / growth & development,  isolation & purification*
Nasopharynx / virology*
Paramyxoviridae Infections / diagnosis,  virology*
Prospective Studies
Respiratory Tract Infections / diagnosis,  virology*
Time Factors
Virus Cultivation*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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