Document Detail


Comparison of different antibody preparations against pregnancy-associated plasma protein-A (PAPP-A) for use in localization and immunoassay studies.
MedLine Citation:
PMID:  2429686     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Four antibody preparations against pregnancy-associated plasma protein (PAPP-A) were compared in order to find an explanation for the contradictory results published on tissue localization, clinical usefulness and biological function of PAPP-A. One of the preparations studied was a rabbit anti-PAPP-A antiserum which has been offered for general scientific use (Bischof et al. 1979). Only the IgG fraction of anti-PAPP-A antisera which appeared to be monospecific and had been further absorbed with fetal connective tissue gave specific uniform staining of the cytoplasm of the syncytiotrophoblast exclusively. Circulating PAPP-A could not be detected by RIA employing this IgG preparation in the non-pregnant state, or before 18 days after conception. Circulating PAPP-A could be detected in all seven pregnant women studied within 4 weeks after conception. Identical results were obtained with a commercially available IgG fraction against PAPP-A.
Authors:
J Chemnitz; J Folkersen; B Teisner; M J Sinosich; D Tornehave; J G Westergaard; A E Bolton; J G Grudzinskas
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  British journal of obstetrics and gynaecology     Volume:  93     ISSN:  0306-5456     ISO Abbreviation:  Br J Obstet Gynaecol     Publication Date:  1986 Sep 
Date Detail:
Created Date:  1986-12-17     Completed Date:  1986-12-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7503752     Medline TA:  Br J Obstet Gynaecol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  916-23     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology*
Antibody Specificity
Humans
Immunoelectrophoresis
Immunoenzyme Techniques
Pregnancy Proteins / immunology*
Pregnancy-Associated Plasma Protein-A / immunology*
Rabbits
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Pregnancy Proteins; EC 3.4.24.-/Pregnancy-Associated Plasma Protein-A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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