Document Detail

Comparison of diethyl ether and ethyl acetate as extracting agents for recovery of Ascaris spp. and Trichuris spp. eggs.
MedLine Citation:
PMID:  3436889     Owner:  NLM     Status:  MEDLINE    
Ethyl acetate and diethyl ether were compared for their ability to recover Ascaris spp. and Trichuris spp. eggs from seeded milorganite, liquid sludge, and cabbage. Concentrations of 10, 100, and 1000 eggs/10 g test sample were prepared for 20 replicates of each product. The use of diethyl ether yielded fewer eggs/10 g than did ethyl acetate in 5 of 6 sets of data. For Ascaris spp., recovery from cabbage was 10 times higher with ethyl acetate at the higher concentration than with diethyl ether. For Trichuris spp., recovery from liquid sludge was slightly higher with diethyl ether for all egg concentrations. The other results ranged from 0 to 23% difference in recovery for the 2 agents. Depending on the parasites in question and the products to be screened, the substitution of ethyl acetate for diethyl ether may be significant.
R A Rude; J T Peeler; N G Risty
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal - Association of Official Analytical Chemists     Volume:  70     ISSN:  0004-5756     ISO Abbreviation:  J Assoc Off Anal Chem     Publication Date:    1987 Nov-Dec
Date Detail:
Created Date:  1988-03-25     Completed Date:  1988-03-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7505559     Medline TA:  J Assoc Off Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1000-2     Citation Subset:  IM    
Food and Drug Administration, Center for Microbiological Investigations, Minneapolis, MN 55401.
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MeSH Terms
Acetic Acids*
Ascaris / analysis*
Ether, Ethyl*
Ethyl Ethers*
Food Contamination / analysis
Indicators and Reagents
Ovum / analysis*
Trichuris / analysis*
Vegetables / analysis
Reg. No./Substance:
0/Acetates; 0/Acetic Acids; 0/Ethyl Ethers; 0/Indicators and Reagents; 0/Solvents; 141-78-6/ethyl acetate; 60-29-7/Ether, Ethyl

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