Document Detail


Comparison of development and implantation of human embryos biopsied with two different methods: aspiration and displacement.
MedLine Citation:
PMID:  18692831     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To compare the development and implantation of human embryos biopsied with two different methods for preimplantation genetic diagnosis (PGD). DESIGN: Technique and method. SETTING: A regional hospital IVF laboratory and private reproductive medicine clinic. PATIENT(S): Women undergoing IVF and PGD. INTERVENTION(S): Day 3 embryos were biopsied with aspiration and displacement; the embryos were cultured to blastocyst stage and then transferred. MAIN OUTCOME MEASURE(S): Blastocyst rate, pregnancy rate, and implantation rate. RESULT(S): One hundred fifty-one embryos from 14 patients were biopsied with the blastomere displacement method and 51 embryos from 5 patients were biopsied with the aspiration method. Displacement used less time than aspiration; thus, the time of embryos exposed to biopsy solution was shorter when displacement was used. Blastocyst formation (55.6%-56.8%) and ongoing pregnancy rate (50%) were not different between the two biopsy methods. However, the implantation rate was significantly higher in patients with embryos biopsied using the displacement method (64.7%) than with the aspiration method (25%). CONCLUSION(S): Blastomere displacement uses less time and is an easy and simple method for embryo biopsy and could be used as an alternative method for embryo biopsy. Our results indicate that the displacement method minimizes embryo damage during biopsy that was indicated by a higher implantation rate.
Authors:
Wei-Hua Wang; Khalied Kaskar; Yuhong Ren; Jimmy Gill; Traci DeSplinter; Ghassan Haddad; Manvinder Singh
Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2008-08-09
Journal Detail:
Title:  Fertility and sterility     Volume:  92     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-03     Completed Date:  2009-08-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  536-40     Citation Subset:  IM    
Affiliation:
Houston Fertility Institute/Tomball Regional Hospital, Tomball, Texas 77375, USA. wangweihua11@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Adult
Biopsy / methods*
Embryo Transfer / methods*
Embryo, Mammalian / cytology*
Female
Humans
Infertility, Female / therapy*
Pregnancy
Pregnancy Outcome*
Preimplantation Diagnosis / methods*
Treatment Outcome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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