| Comparison of conditioned medium and direct co-culture of human granulosa cells on mouse embryo development. | |
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MedLine Citation:
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PMID: 16538856 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although numerous investigations have demonstrated the beneficial effects of co-culture system of different somatic cells on in vitro development of embryos, the effects of conditioned-media of co-culture cells have not been well documented. The objective of this study was to compare the effects of human granulosa cells co-culture system and its conditioned medium on the developmental rate of mouse embryos in vitro. Two sets of experiments were undertaken: in the first one 317 mouse one-cell embryos were cultured in human granulosa cell co-culture system (GC). Ham's F10 medium conditioned with granulosa cells (CM) and non-conditioned Ham's F10 for 120 h. In the second experiment. 391 late two-cell embryos were cultured in the 3 fore-mentioned culture treatments for 72 h. Embryos were obtained from NMRI mice. Granulosa cells were collected from patients undergoing an IVF program during oocyte pickup. In the first set of experiments, 23.6, 14.5 and 11.1% of one-cell embryos passed two-cell block and continued growing to 4-cell in GC, CM and HF, respectively. This index in GC was significantly different from two other treatments. Also significantly more embryos reached blastocyst stage in GC compared with two other treatments. The blastocyst rate was not significantly different between CM and HF. In the second set of experiments the proportion of blastocyst stage was significantly higher in CM than that in HF and lower than that in GC. In conclusion, although human granulosa cell-conditioned medium has beneficial effects on mouse embryo development, it was not as effective as co-culture of these cells. |
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Authors:
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Abbasali Karimpour Malekshah; Amir Esmailnejad Moghaddam; Saeed Musavi Daraka |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Indian journal of experimental biology Volume: 44 ISSN: 0019-5189 ISO Abbreviation: Indian J. Exp. Biol. Publication Date: 2006 Mar |
Date Detail:
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Created Date: 2006-03-16 Completed Date: 2006-08-21 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0233411 Medline TA: Indian J Exp Biol Country: India |
Other Details:
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Languages: eng Pagination: 189-92 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Embryology, Faculty of Medicine, Mazandaran University of Medical Sciences, Khazar Boulevard, Sari, Iran. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Proliferation / drug effects Coculture Techniques / methods* Culture Media, Conditioned / pharmacology* Embryo, Mammalian / cytology, drug effects*, embryology* Female Granulosa Cells / cytology*, physiology* Humans Ice Mice |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 0/Ice |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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