Document Detail


Comparison of components of the testis interstitium with testosterone secretion in hamster, rat, and guinea pig testes perfused in vitro.
MedLine Citation:
PMID:  3348144     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Components of the testis and cytoplasmic organelles in Leydig cells were quantified with morphometric techniques in hamster, rat, and guinea pig. Testosterone secretory capacity per gram of testis and per Leydig cell in response to luteinizing hormone (LH) (100 ng/ml) stimulation was determined in these three species from testes perfused in vitro. Numerous correlations were measured among structures, and between structures and testosterone secretion, to provide structural evidence of intratesticular control of Leydig cell function. Testosterone secretion per gm testis and per Leydig cell was significantly different in the three species: highest in the guinea pig, intermediate in the rat, and lowest in the hamster. The volume of seminiferous tubules per gm testis was negatively correlated, and the volumes of interstitium, Leydig cells, and lymphatic space per gm testis were positively correlated with testosterone secretion. No correlations were observed between volumes of blood vessels, elongated spindleshaped cells, or macrophages per gm testes and testosterone secretion. The average volume of a Leydig cell and the volume and surface area of smooth endoplasmic reticulum (SER) and peroxisomes per Leydig cell were positively correlated, and the volume of lysosomes and surface area of inner mitochondrial membrane per Leydig cell were negatively correlated with testosterone secretion. No correlations were observed between volume and surface area of rough endoplasmic reticulum (RER), Golgi apparatus, and lipid, and volume of ribosomes, cytoplasmic matrix, and the nucleus with testosterone secretion per Leydig cell. These results suggest that Leydig cell size is more important than number of Leydig cells in explaining the difference in testosterone-secreting capacity among the three species, and that this increase in average volume of a Leydig cell is associated specifically with increased volume and surface area of SER and peroxisomes. An important unresolved question is what is the role of peroxisomes in Leydig cell steroidogenesis.
Authors:
S M Mendis-Handagama; B R Zirkin; L L Ewing
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of anatomy     Volume:  181     ISSN:  0002-9106     ISO Abbreviation:  Am. J. Anat.     Publication Date:  1988 Jan 
Date Detail:
Created Date:  1988-04-15     Completed Date:  1988-04-15     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0376312     Medline TA:  Am J Anat     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  12-22     Citation Subset:  IM    
Affiliation:
Department of Population Dynamics, Johns Hopkins School of Hygiene and Public Health, Baltimore, Maryland 21205.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Cricetinae
Guinea Pigs
Leydig Cells / ultrastructure
Luteinizing Hormone / pharmacology
Male
Rats
Statistics as Topic
Testis / ultrastructure*
Testosterone / secretion*
Grant Support
ID/Acronym/Agency:
2-P30-HD-06268/HD/NICHD NIH HHS; HD-07204/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
58-22-0/Testosterone; 9002-67-9/Luteinizing Hormone
Comments/Corrections
Erratum In:
Am J Anat 1989 Dec;186(4):430

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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