Document Detail


Comparison of a commercial ELISA with the modified agglutination test for the detection of Toxoplasma gondii infection in naturally exposed sheep.
MedLine Citation:
PMID:  17456149     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Surprisingly few commercial ELISAs are available for the detection of Toxoplasma gondii infection in animals, and none for use in sheep have been evaluated. We thus compared the Bommeli Diagnostics ELISA Toxotest for the detection of T. gondii antibodies in ruminants with the reference modified agglutination test (MAT) in a series of 180 sheep sera. ELISA results were analysed at two cut-off levels (30%, comprising both weakly positive and positive results, and 100%, comprising only positive results), and compared with MAT at three cut-off levels (titre of 1 : 25, 1 : 50 and 1 : 100). The results showed a moderate agreement of ELISA at both cut-offs (kappa = 0.46 and 0.51) with MAT at a cut-off titre of 1 : 100. However, the specificity and positive predictive value were above 95% only at an ELISA cut-off of 100%, indicating its potential as a diagnostic test, particularly in areas with a high prevalence of infection. On the other hand, lower sensitivity and negative predictive value limit its value as a screening test. Thus, the ELISA Toxotest may be used for quick diagnosis of T. gondii infection in sheep in the field, i.e. for the differential diagnosis of ovine abortion storms.
Authors:
I Klun; O Djurković-Djaković; P Thulliez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zoonoses and public health     Volume:  54     ISSN:  1863-1959     ISO Abbreviation:  Zoonoses Public Health     Publication Date:  2007  
Date Detail:
Created Date:  2007-04-25     Completed Date:  2007-06-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101300786     Medline TA:  Zoonoses Public Health     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  165-8     Citation Subset:  IM    
Affiliation:
Department of Parasitology, Institute for Medical Research, University of Belgrade, PO Box 102, 11129 Belgrade, Serbia.
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MeSH Terms
Descriptor/Qualifier:
Agglutination Tests / methods,  veterinary*
Animals
Antibodies, Protozoan / blood*
Diagnosis, Differential
Dose-Response Relationship, Immunologic
Enzyme-Linked Immunosorbent Assay / methods,  veterinary*
Predictive Value of Tests
Public Health
Sensitivity and Specificity
Seroepidemiologic Studies
Sheep
Sheep Diseases / diagnosis*,  epidemiology,  transmission
Toxoplasma / immunology
Toxoplasmosis, Animal / diagnosis*,  epidemiology,  transmission
Yugoslavia / epidemiology
Chemical
Reg. No./Substance:
0/Antibodies, Protozoan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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