Document Detail


Comparison of coliforms and coliphages as tools for assessment of viral contamination in river water.
MedLine Citation:
PMID:  15184169     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of the study was to evaluate the presence of pathogenic viruses in the Moselle River and to compare the usefulness of thermotolerant coliforms and somatic coliphages as tools for river water quality assessment in terms of viral contamination. Thermotolerant coliforms and somatic coliphages were enumerated by standardized methods in 170 samples of river water drawn from five sampling sites along the Moselle River (eastern France). BGM cell culture and integrated cell culture-reverse transcription-PCR DNA enzyme immunoassay were used to determine the presence of pathogenic viral genome (Enterovirus and Norovirus genogroup II [GGII]) and infectious Enterovirus spp. in 90 1-liter samples. No infectious Enterovirus spp. were isolated, but Enterovirus and Norovirus GGII genomes were detected in 38% of the samples. Norovirus GGII genome was mostly detected in winter, whereas Enterovirus genome was mostly detected in summer and fall. Somatic coliphages appeared to be less sensitive to higher river water temperature than thermotolerant coliforms. Furthermore, the number of river water samples positive for pathogenic viral genome increased with increasing concentration of somatic coliphages, whereas coliform concentration was unrelated to viral genome contamination. Consequently somatic coliphages, which are less sensitive to environmental factors than thermotolerant coliforms in river water, would provide a promising tool for assessment of river water quality in terms of fecal and viral pollution.
Authors:
S Skraber; B Gassilloud; C Gantzer
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  70     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-06-08     Completed Date:  2004-08-10     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3644-9     Citation Subset:  IM    
Affiliation:
LCPME-UMR 7564 CNRS/UHP, Virologie, Faculté de Pharmacie, 54001 Nancy, France.
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MeSH Terms
Descriptor/Qualifier:
Coliphages / genetics,  isolation & purification*
Colony Count, Microbial
Enterobacteriaceae / genetics,  isolation & purification*
Enterovirus / genetics,  isolation & purification,  pathogenicity
Environmental Monitoring / methods*
Feces / microbiology,  virology
Hot Temperature
Humans
Norovirus / genetics,  isolation & purification,  pathogenicity
Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Rivers / microbiology*
Virus Cultivation
Water Pollution*
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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