Document Detail

Comparison of clinical diagnosis and standard laboratory and molecular methods for the diagnosis of genital ulcer disease in Lesotho: association with human immunodeficiency virus infection.
MedLine Citation:
PMID:  9041329     Owner:  NLM     Status:  MEDLINE    
A multiplex polymerase chain reaction (M-PCR) assay for Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus (HSV) was compared with clinical and standard laboratory methods for the diagnosis of genital ulcer disease (GUD) in 105 patients; 36% were human immunodeficiency virus (HIV)-seropositive. Chancroid (80%), syphilis (8%), and genital herpes (8%) were the most frequent diagnoses. H. ducreyi and HSV were isolated from ulcers of 43% and 18% of patients, respectively; in 35%, all cultures were negative and the laboratory diagnosis indeterminate. M-PCR detected H. ducreyi, T. pallidum, and HSV in 56%, 23%, and 26% of patients, respectively; (no definitive diagnosis, 6%). The proportion of patients with more than one agent was 4% by culture and 17% by M-PCR (P = .002). Resolved sensitivities of M-PCR for H. ducreyi and HSV cultures were 95% and 93%, respectively. The sensitivities of H. ducreyi and HSV cultures were 75% and 60%, respectively. HSV, detected in 47% of specimens from HIV-infected versus 16% from HIV-uninfected patients (P < .001), may be emerging as a more frequent cause of GUD.
S A Morse; D L Trees; Y Htun; F Radebe; K A Orle; Y Dangor; C M Beck-Sague; S Schmid; G Fehler; J B Weiss; R C Ballard
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  175     ISSN:  0022-1899     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-03-20     Completed Date:  1997-03-20     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  583-9     Citation Subset:  AIM; IM; X    
Division of AIDS, Sexually Transmitted Diseases and Tuberculosis Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
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MeSH Terms
Antibodies, Viral / analysis
Chancroid / complications,  diagnosis
DNA, Bacterial / analysis
DNA, Viral / analysis
Genital Diseases, Female / diagnosis*,  microbiology
Genital Diseases, Male / diagnosis*,  microbiology
HIV Infections / complications,  diagnosis*,  microbiology
Haemophilus ducreyi / genetics
Herpes Genitalis / immunology
Middle Aged
Polymerase Chain Reaction / methods
Simplexvirus / genetics,  immunology
Syphilis / complications,  diagnosis
Treponema pallidum / genetics
Ulcer / complications,  diagnosis*,  microbiology
Reg. No./Substance:
0/Antibodies, Viral; 0/DNA, Bacterial; 0/DNA, Viral

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