Document Detail


Comparison of chromosome preparation methods for the different developmental stages of the half-smooth tongue sole, Cynoglossus semilaevis.
MedLine Citation:
PMID:  19781952     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Here we present a comparison of the different chromosome preparation methods, including the method of the single embryo or larva, the method of juvenile-swimming and the method of phytohemagglutinin injection, used for the different developmental stages of the half-smooth tongue sole, Cynoglossus semilaevis. The mean index of mitosis for the three methods was 0.79%, 0.09% and 0.15%, respectively. From the well-spread metaphase chromosomes obtained, it was determined that C. semilaevis has a diploid chromosome number of 42 and heterotypic sex chromosomes. Also, the metaphase chromosomes produced could be used for karyotype preparation and banding studies. The three methods are also successfully used for chromosome preparation in Japanese flounder (Paralichthys olivaceus) and summer flounder (Paralichthys dentatus).
Authors:
Chang-Wei Shao; Peng-Fei Wu; Xian-Li Wang; Yong-Sheng Tian; Song-Lin Chen
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-12
Journal Detail:
Title:  Micron (Oxford, England : 1993)     Volume:  41     ISSN:  1878-4291     ISO Abbreviation:  Micron     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-11-05     Completed Date:  2010-01-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9312850     Medline TA:  Micron     Country:  England    
Other Details:
Languages:  eng     Pagination:  47-50     Citation Subset:  IM    
Affiliation:
Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, Shandong 266071, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromosome Banding
Chromosomes*
Cytogenetic Analysis / methods*
Diploidy
Flatfishes / genetics*
Karyotyping
Metaphase
Mitosis

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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