Document Detail


Comparison of chromogenic Shigella spp. plating medium with standard media for the recovery of Shigella boydii and Shigella sonnei from tomato surfaces.
MedLine Citation:
PMID:  15771194     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Isolation of Shigella spp. from food is difficult because of a lack of appropriate selective media and the presence of low numbers of shigellae relative to competitive microorganisms. Chromogenic Shigella spp. plating medium (CSPM) was evaluated for use with the U.S. Food and Drug Administration Bacteriological Analytical Manual (BAM) enrichment procedure for isolation of artificially contaminated Shigella boydii UI02 and Shigella sonnei UI05 from tomato surfaces. Tomatoes were inoculated with various concentrations of S. boydii UI02 or S. sonnei UI05 and rinsed using a shake-rub-shake procedure. Tomato rinses were enriched overnight according to the BAM procedure and streaked for isolation on CSPM, Salmonella-Shigella agar (SSA), and MacConkey agar (MAC). To access the isolation of S. boydii UI02 and S. sonnei UI05 without competition from natural tomato microflora, experiments were repeated using rifampin-adapted inocula and enrichments supplemented with 50 microg/ml rifampin. Isolation of S. boydii UI02 and S. sonnei UI05 with or without natural tomato microflora was not significantly different (P > 0.05) on CSPM, MAC, or SSA. Colony color enhancements created by CSPM may ease differentiation of Shigella colonies from those of closely related competitors.
Authors:
B R Warren; M E Parish; K R Schneider
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of food protection     Volume:  68     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-17     Completed Date:  2005-04-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  621-4     Citation Subset:  IM    
Affiliation:
Department of Food Science and Human Nutrition, University of Florida, Gainesville, Florida 32611, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacteriological Techniques
Colony Count, Microbial
Culture Media / chemistry*
Food Contamination / analysis*
Food Microbiology*
Lycopersicon esculentum / microbiology*
Shigella / growth & development,  isolation & purification*
Shigella boydii / growth & development,  isolation & purification
Shigella sonnei / growth & development,  isolation & purification
Species Specificity
Chemical
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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