| Comparison of chemiluminescence and flow cytometry in the estimation of reactive oxygen and nitrogen species in human semen. | |
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MedLine Citation:
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PMID: 20413114 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine the relative contribution of different cell types in washed sperm to the overall intracellular production of H(2)O(2) and peroxynitrite. DESIGN: Prospective study. SETTING: University hospital. PATIENT(S): Thirty-one fertile volunteers and 166 men undergoing fertility assessment were included. INTERVENTION(S): Aliquots of sperm suspension in phosphate-buffered saline solution were used for the reactive oxygen species (ROS) detection by chemiluminescence and for the detection of H(2)O(2) and peroxynitrite by flow cytometry, with use of specific fluorescent probes, carboxy-2',7'-dichlorodihydrofluorescein diacetate dye for H(2)O(2) and dihydrorhodamine 123 for peroxynitrite. Gated analysis determined the relative contribution of spermatozoa, leukocytes, and "other round cells." MAIN OUTCOME MEASURE(S): Simultaneous estimates of global ROS production assessed by chemiluminescence assay compared with flow cytometric measurements. RESULT(S): The estimates of ROS with use of chemiluminescence positively correlated with the estimates of H(2)O(2) (r = 0.53) and peroxynitrite (r = 0.62) as assessed with flow cytometry. H(2)O(2) and peroxynitrite were measurable also in samples in which chemiluminescence did not detect measurable values. Increased production of H(2)O(2) by one cell type was associated with a relative increase in its peroxynitrite production. CONCLUSION(S): The levels of ROS production measured by chemiluminescence and flow cytometry were related. Each cell type in semen contributed differently to the global intracellular levels of H(2)O(2) and peroxynitrite. |
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Authors:
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Nabil Aziz; Jiri Novotny; Ivana Oborna; Helena Fingerova; Jana Brezinova; Magda Svobodova |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-22 |
Journal Detail:
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Title: Fertility and sterility Volume: 94 ISSN: 1556-5653 ISO Abbreviation: Fertil. Steril. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-26 Completed Date: 2011-01-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: United States |
Other Details:
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Languages: eng Pagination: 2604-8 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Gynaecology and Reproductive Medicine, Liverpool Women's Hospital and the University of Liverpool, Merseyside, United Kingdom. naziz@liv.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Case-Control Studies Chemiluminescent Measurements / methods* Flow Cytometry / methods* Humans Hydrogen Peroxide / analysis, metabolism Infertility, Male / diagnosis, metabolism Leukocyte Count Male Peroxynitrous Acid / analysis, metabolism Reactive Nitrogen Species / analysis*, metabolism Reactive Oxygen Species / analysis*, metabolism Semen / chemistry*, metabolism Semen Analysis / methods Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Reactive Nitrogen Species; 0/Reactive Oxygen Species; 14691-52-2/Peroxynitrous Acid; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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