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Comparison of the chemical properties of a novel amino-linker with various amino modifications.
MedLine Citation:
PMID:  18776454     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
We previously reported a series of new amino-linkers, consisting of an aminoethyl carbamate structure (Komatsu, 2008). We have now examined the chemical properties of oligonucleotides modified with an ssH-linker, which is the simplest and most cost-effective derivative of the series. Although it was previously shown that monomethoxytrityl protection on a primary amine of the ssH-linker was cleaved under weakly acidic conditions (1% acetic acid), we found that the deprotection also proceeded in aqueous buffer solutions (pH 6.0, 7.0). The MMT group was removed much faster than other commercially available amino-linkers, and this property enabled the ssH-modified oligonucleotides to be conveniently purified with a cartridge column. Furthermore, the ssH-modified oligonucleotides were utilized in on-support labeling reactions. As compared with other amino-linkers, the ssH-linker was superior in terms of its purification and reaction efficiencies.
Authors:
Naoshi Kojima; Toshie Takebayashi; Akiko Mikami; Maiko Sugino; Eiko Ohtsuka; Yasuo Komatsu
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Nucleic acids symposium series (2004)     Volume:  -     ISSN:  1746-8272     ISO Abbreviation:  Nucleic Acids Symp Ser (Oxf)     Publication Date:  2008  
Date Detail:
Created Date:  2008-09-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101259965     Medline TA:  Nucleic Acids Symp Ser (Oxf)     Country:  England    
Other Details:
Languages:  eng     Pagination:  463-4     Citation Subset:  IM    
Affiliation:
Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science & Technology (AIST Hokkaido), Toyohira-ku, Sapporo 062-8517, Japan.
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