Document Detail

Comparison of cellular damage response to low-dose-rate (125)I seed irradiation and high-dose-rate gamma irradiation in human lung cancer cells.
MedLine Citation:
PMID:  21664878     Owner:  NLM     Status:  Publisher    
PURPOSE: To investigate the difference of cellular response between low-dose-rate (LDR) (125)I seed irradiation and high-dose-rate (HDR) γ-irradiation in human lung cancer cells. METHODS AND MATERIALS: A549 and NCI-H446 cells with or without wortmannin (WM) treatment were exposed to (125)I seeds and γ-rays, respectively. Cell survival, micronuclei (MN) formation, and the expressions of Ku70/Ku80 proteins were measured. RESULTS: There was a strong negative correlation between survival and MN formation for both irradiations, and the MN inductions of NCI-H446 were about twofolds of those of A549, and the survival of NCI-H446 was lower than that of A549, indicating the radiosensitivity of NCI-H446 cells was greater than that of A549 cells. Interestingly, at 4-Gy radiation, NCI-H446 cells were more sensitive to LDR irradiation than HDR irradiation. WM treatment enhanced the radiosensitivity of A549 cells evenly to (125)I seed and γ-irradiation, but this treatment led NCI-H446 cells to be more sensitive to LDR (125)I. Further results revealed that the expression of phosphorylated Ku80 protein was enhanced in irradiated A549, but in contrast, it was markedly decreased in NCI-H446 cells after 4-Gy LDR (125)I irradiation as that compared with γ-irradiated and nonirradiated cells. CONCLUSION: NCI-H446 cells were more sensitive to LDR (125)I irradiation than HDR irradiation, and this sensitivity could be further enhanced by WM treatment. But no obvious differences of cellular response to both irradiations were observed in A549. Ku as molecular markers together with cell proliferation rate can be used to predict the radiosensitivity of tumor cells to LDR (125)I seed irradiation.
Honghong Chen; Yizhong Bao; Lei Yu; Rongfei Jia; Wenying Cheng; Chunlin Shao
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-6-10
Journal Detail:
Title:  Brachytherapy     Volume:  -     ISSN:  1873-1449     ISO Abbreviation:  -     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-6-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101137600     Medline TA:  Brachytherapy     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 American Brachytherapy Society. Published by Elsevier Inc. All rights reserved.
Department of Radiation Biology, Institute of Radiation Medicine, Fudan University, Shanghai, P. R. China.
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