Document Detail


Comparison of cellular composition of induced sputum analyzed with Wright staining and immunocytochemistry.
MedLine Citation:
PMID:  11590375     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We sought to compare cell counts based on cellular morphology and obtained after Wright staining with cell counts obtained after immunocytochemistry in induced sputum. Counts of eosinophils, neutrophils, macrophages, lymphocytes, and epithelial cells identified after Wright staining were compared with the counts obtained after immunocytochemistry through use of mAbs in 25 samples. Agreement between Wright staining and immunocytochemistry was poor for lymphocytes and epithelial cells. Rather than Wright staining, immunocytochemistry should be used to accurately identify lymphocytes and epithelial cells.
Authors:
C Lemière; R Taha; R Olivenstein; Q Hamid
Related Documents :
11027175 - Biomass and aggregation analysis of human embryonic kidney 293 suspension cell cultures...
8946015 - A simple and rapid method for removal of specific cell populations from whole blood.
11835415 - A new continuous-flow cell separation method based on cell density: principle, apparatu...
1629615 - A novel b cell stimulation/proliferation assay using simultaneous flow cytometric detec...
21979235 - Generation of ips cells using a bacmam multigene expression system.
19858255 - Pretreatment of epithelial cells with rifaximin alters bacterial attachment and interna...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of allergy and clinical immunology     Volume:  108     ISSN:  0091-6749     ISO Abbreviation:  J. Allergy Clin. Immunol.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-10-08     Completed Date:  2001-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1275002     Medline TA:  J Allergy Clin Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  521-3     Citation Subset:  AIM; IM    
Affiliation:
Department of Chest Medicine, Hôpital du Sacré-Coeur, Montral, Quebec, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Asthma / pathology*
Cell Count / methods*
Humans
Immunohistochemistry
Occupational Diseases / pathology*
Sputum / cytology*
Staining and Labeling

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Allergy development and the intestinal microflora during the first year of life.
Next Document:  Distinct pattern of immune cell population in the lung of human fetuses with cystic fibrosis.