Document Detail

Comparison of cellular composition of induced sputum analyzed with Wright staining and immunocytochemistry.
MedLine Citation:
PMID:  11590375     Owner:  NLM     Status:  MEDLINE    
We sought to compare cell counts based on cellular morphology and obtained after Wright staining with cell counts obtained after immunocytochemistry in induced sputum. Counts of eosinophils, neutrophils, macrophages, lymphocytes, and epithelial cells identified after Wright staining were compared with the counts obtained after immunocytochemistry through use of mAbs in 25 samples. Agreement between Wright staining and immunocytochemistry was poor for lymphocytes and epithelial cells. Rather than Wright staining, immunocytochemistry should be used to accurately identify lymphocytes and epithelial cells.
C Lemière; R Taha; R Olivenstein; Q Hamid
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of allergy and clinical immunology     Volume:  108     ISSN:  0091-6749     ISO Abbreviation:  J. Allergy Clin. Immunol.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-10-08     Completed Date:  2001-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1275002     Medline TA:  J Allergy Clin Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  521-3     Citation Subset:  AIM; IM    
Department of Chest Medicine, Hôpital du Sacré-Coeur, Montral, Quebec, Canada.
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MeSH Terms
Asthma / pathology*
Cell Count / methods*
Occupational Diseases / pathology*
Sputum / cytology*
Staining and Labeling

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