| Comparison of cellular composition of induced sputum analyzed with Wright staining and immunocytochemistry. | |
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MedLine Citation:
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PMID: 11590375 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We sought to compare cell counts based on cellular morphology and obtained after Wright staining with cell counts obtained after immunocytochemistry in induced sputum. Counts of eosinophils, neutrophils, macrophages, lymphocytes, and epithelial cells identified after Wright staining were compared with the counts obtained after immunocytochemistry through use of mAbs in 25 samples. Agreement between Wright staining and immunocytochemistry was poor for lymphocytes and epithelial cells. Rather than Wright staining, immunocytochemistry should be used to accurately identify lymphocytes and epithelial cells. |
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Authors:
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C Lemière; R Taha; R Olivenstein; Q Hamid |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of allergy and clinical immunology Volume: 108 ISSN: 0091-6749 ISO Abbreviation: J. Allergy Clin. Immunol. Publication Date: 2001 Oct |
Date Detail:
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Created Date: 2001-10-08 Completed Date: 2001-12-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1275002 Medline TA: J Allergy Clin Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 521-3 Citation Subset: AIM; IM |
Affiliation:
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Department of Chest Medicine, Hôpital du Sacré-Coeur, Montral, Quebec, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Asthma
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pathology* Cell Count / methods* Humans Immunohistochemistry Occupational Diseases / pathology* Sputum / cytology* Staining and Labeling |
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