Document Detail


Comparison between therapeutic antitoxin F(ab)2 fractionated with ammonium sulfate and caprylic acid.
MedLine Citation:
PMID:  16981645     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To date, animal derived therapeutic antibodies represent the best and only choice source of antitoxins, especially in developing countries. Furthermore, this industry needs to develop a production protocol to achieve safer products. Recently, several laboratories changed their production protocol from ammonium sulfate (AS) protocol to caprylic acid (CA) fractionation. Our results showed that using the CA protocol leads to improvement in the product quality, as assessed by the albumin and protein content decrease (from 4.75 to 3.54 g/dL and 0.64 to 0.18 g/dL, respectively), which yielded a purer antitoxin product. The F(ab)2 protein aggregate formation and turbidity have been significantly reduced, 4.60 versus 2.55 and 0.046 versus 0.021 (p < 0.01), respectively. However, the anti-complementary activity was also reduced, from 42 to 33. The total IgG content was higher in CA fractionated products than AS materials. The endotoxin content was worrisome in some F(ab)2 products.
Authors:
El-Rashdy M Redwan
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunoassay & immunochemistry     Volume:  27     ISSN:  1532-1819     ISO Abbreviation:  J Immunoassay Immunochem     Publication Date:  2006  
Date Detail:
Created Date:  2006-09-19     Completed Date:  2006-10-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100963688     Medline TA:  J Immunoassay Immunochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  319-29     Citation Subset:  IM    
Affiliation:
Antibody Laboratory, Protein Research Department, GEBRI, Mubarak City for Scientific Research and Technology Applications, New Borg EL Arab, 21394, Alexandria, Egypt. redwan1961@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Ammonium Sulfate / chemistry*
Antigen-Antibody Reactions
Antitoxins / chemistry*,  isolation & purification
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Fractional Precipitation
Humans
Immunoglobulin Fab Fragments / chemistry*,  isolation & purification
Lipopolysaccharides / chemistry
Octanoic Acids / chemistry*
Quality Control
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Antitoxins; 0/Immunoglobulin Fab Fragments; 0/Lipopolysaccharides; 0/Octanoic Acids; 124-07-2/caprylic acid; 7783-20-2/Ammonium Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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