Document Detail

Comparison between therapeutic antitoxin F(ab)2 fractionated with ammonium sulfate and caprylic acid.
MedLine Citation:
PMID:  16981645     Owner:  NLM     Status:  MEDLINE    
To date, animal derived therapeutic antibodies represent the best and only choice source of antitoxins, especially in developing countries. Furthermore, this industry needs to develop a production protocol to achieve safer products. Recently, several laboratories changed their production protocol from ammonium sulfate (AS) protocol to caprylic acid (CA) fractionation. Our results showed that using the CA protocol leads to improvement in the product quality, as assessed by the albumin and protein content decrease (from 4.75 to 3.54 g/dL and 0.64 to 0.18 g/dL, respectively), which yielded a purer antitoxin product. The F(ab)2 protein aggregate formation and turbidity have been significantly reduced, 4.60 versus 2.55 and 0.046 versus 0.021 (p < 0.01), respectively. However, the anti-complementary activity was also reduced, from 42 to 33. The total IgG content was higher in CA fractionated products than AS materials. The endotoxin content was worrisome in some F(ab)2 products.
El-Rashdy M Redwan
Related Documents :
680085 - Collagen-bound glycoprotein of guerin epithelioma.
22293415 - Cattle bile but not bear bile or pig bile induces lipid profile changes and fatty liver...
7326065 - Proteoglycan structure and metabolism in normal and osteoarthritic cartilage of guinea ...
22665165 - Metabolomics reveals an essential role for peroxisome proliferator-activated receptor ...
1629295 - Liquid chromatography-mass spectrometry of hydroxy and non-hydroxy fatty acids as amide...
6313435 - Leukocyte recognition and metabolism of leukotrienes.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunoassay & immunochemistry     Volume:  27     ISSN:  1532-1819     ISO Abbreviation:  J Immunoassay Immunochem     Publication Date:  2006  
Date Detail:
Created Date:  2006-09-19     Completed Date:  2006-10-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100963688     Medline TA:  J Immunoassay Immunochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  319-29     Citation Subset:  IM    
Antibody Laboratory, Protein Research Department, GEBRI, Mubarak City for Scientific Research and Technology Applications, New Borg EL Arab, 21394, Alexandria, Egypt.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Ammonium Sulfate / chemistry*
Antigen-Antibody Reactions
Antitoxins / chemistry*,  isolation & purification
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Fractional Precipitation
Immunoglobulin Fab Fragments / chemistry*,  isolation & purification
Lipopolysaccharides / chemistry
Octanoic Acids / chemistry*
Quality Control
Sensitivity and Specificity
Reg. No./Substance:
0/Antitoxins; 0/Immunoglobulin Fab Fragments; 0/Lipopolysaccharides; 0/Octanoic Acids; 124-07-2/caprylic acid; 7783-20-2/Ammonium Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Production of anti-digoxigenin antibody HRP conjugate for PCR-ELISA DIG detection system.
Next Document:  Detection of serum antibodies to S-antigen by surface plasmon resonance (SPR).