Document Detail


Comparison between radioreceptor assay and radioimmunoassay for the determination of dihydroergotoxine in rabbit plasma samples.
MedLine Citation:
PMID:  6655239     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two analytical methods, radioreceptor assay and radioimmunoassay, for the determination of dihydroergotoxine have been developed. Antiserum, providing sufficient sensitivity for the radioimmunoassay, was produced by immunizing rabbits with D-lysergic acid coupled to bovine serum albumin. Radioreceptor assay utilizing dopamine receptor was carried out to determine dihydroergotoxine and its pharmacologically active metabolites in rabbit plasma, and the result was compared with that obtained by radioimmunoassay. The values obtained in both assays were almost identical; it was, therefore, assumed that the plasma concentrations of dihydroergotoxine determined by the present radioimmunoassay reflects the amount of unchanged drug and its active metabolites.
Authors:
N Shimada; K Ushioda; S Nagatsuka; T Ueda; T Yokoshima
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of immunological methods     Volume:  65     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-02-14     Completed Date:  1984-02-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  191-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cross Reactions
Dihydroergotoxine / blood*,  pharmacology
Dose-Response Relationship, Drug
Dose-Response Relationship, Immunologic
Male
Rabbits
Radioimmunoassay / methods*
Radioligand Assay / methods*
Rats
Rats, Inbred Strains
Receptors, Dopamine / drug effects
Chemical
Reg. No./Substance:
0/Receptors, Dopamine; 11032-41-0/Dihydroergotoxine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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