Document Detail


Comparison between normal and loose fragment chondrocytes in proliferation and redifferentiation potential.
MedLine Citation:
PMID:  23197301     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Loose fragments in osteochondritis dissecans (OCD) of the knee require internal fixation. On the other hand, loose fragments derived from spontaneous osteonecrosis of the knee (SONK) are usually removed. However, the difference in healing potential between OCD- and SONK-related loose fragments has not been elucidated. In this study, we investigated proliferative activity and redifferentiation potential of normal cartilage-derived and loose fragment-derived chondrocytes.
METHODS: Cells were prepared from normal articular cartilages and loose fragment cartilages derived from knee OCD and SONK. Cellular proliferation was compared. Redifferentiation ability of pellet-cultured chondrocytes was assessed by real-time PCR analyses. Mesenchymal differentiation potential was investigated by histological analyses. Positive ratio of a stem cell marker CD166 was evaluated in each cartilaginous tissue.
RESULTS: Normal and OCD chondrocytes showed a higher proliferative activity than SONK chondrocytes. Chondrogenic pellets derived from normal and OCD chondrocytes produced a larger amount of safranin O-stained proteoglycans compared with SONK-derived pellets. Expression of chondrogenic marker genes was inferior in SONK pellets. The CD166-positive ratio was higher in normal cartilages and OCD loose fragments than in SONK loose fragments.
CONCLUSIONS: The OCD chondrocytes maintained higher proliferative activity and redifferentiation potential compared with SONK chondrocytes. Our results suggest that chondrogenic properties of loose fragment-derived cells and the amount of CD166-positive cells may affect the repair process of osteochondral defects.
Authors:
Kenichiro Sakata; Takayuki Furumatsu; Shinichi Miyazawa; Yukimasa Okada; Masataka Fujii; Toshifumi Ozaki
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-30
Journal Detail:
Title:  International orthopaedics     Volume:  37     ISSN:  1432-5195     ISO Abbreviation:  Int Orthop     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-31     Completed Date:  2013-05-27     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  7705431     Medline TA:  Int Orthop     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  159-65     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Aged
Analysis of Variance
Antigens, CD / metabolism
Cartilage, Articular / cytology,  metabolism
Cell Adhesion Molecules, Neuronal / metabolism
Cell Differentiation
Cell Proliferation
Cells, Cultured
Chondrocytes / cytology*,  metabolism
Female
Fetal Proteins / metabolism
Humans
Knee Joint / metabolism*
Male
Middle Aged
Osteochondritis Dissecans / metabolism*
Osteonecrosis / metabolism*
Real-Time Polymerase Chain Reaction
Young Adult
Chemical
Reg. No./Substance:
0/ALCAM protein, human; 0/Antigens, CD; 0/Cell Adhesion Molecules, Neuronal; 0/Fetal Proteins
Comments/Corrections

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