| Comparison between multiparameter flow cytometry and WT1-RNA quantification in monitoring minimal residual disease in acute myeloid leukemia without specific molecular targets. | |
| | |
MedLine Citation:
|
PMID: 22196957 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Despite a high remission rate, a significant number of patients with acute myeloid leukemia (AML) relapse. Thus, the evaluation of minimal residual disease (MRD) in AML is an important strategy to better identify high risk patients. Most sensitive methodology to detect MRD is molecular polymerase chain reaction (PCR) but its applicability is restricted to AML with leukemia-specific molecular targets (e.g. AML1-ETO, CBFB-MYH11, MLL, FLT-3). In our study, MRD was monitored at different time points with both MFC and WT1-RNA quantification in 23 AML patients who did not present specific molecular targets. As previously published, we considered values of 10(-3) (0.1%) in MFC and 90 WT1-RNA ×10(4) ABL copies as optimal thresholds. Receiver operating characteristics (ROC) analysis was used to confirm these data. To realize the methodology that better identify high risk patients, an analysis of sensitivity, specificity, predictive values (PV) and likelihood ratio (LR) was provided and similar results were showed. MRD levels ≥10(-3) in MFC as well MRD levels ≥90 WT1-RNA copies in RQ-PCR, identify risk groups of patients with poor prognosis. Therefore, MFC and WT1-RNA quantification showed a comparable capacity in terms of technical performance and clinical significance to identify high risk patients who eventually relapsed. |
| | |
Authors:
|
Giovanni Rossi; Maria Marta Minervini; Angelo Michele Carella; Chiara de Waure; Francesco di Nardo; Lorella Melillo; Giovanni D'Arena; Gina Zini; Nicola Cascavilla |
Related Documents
:
|
22547997 - The rapid teg α-angle may be a sensitive predictor of transfusion in moderately injure... 17898277 - Electrophysiological evidence for heterogeneity of lesions in optic neuritis. 8750557 - Electrophysiological brainstem investigations in obstructive sleep apnoea syndrome. 17226857 - Impaired motor imagery in patients with essential tremor: a case control study. 14506487 - Body mass index and disease duration are predictors of disturbed bone turnover in anore... 7558087 - Late rhythm abnormalities after surgical repair of tetralogy of fallot: noninvasive and... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-12-21 |
Journal Detail:
|
Title: Leukemia research Volume: - ISSN: 1873-5835 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-26 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7706787 Medline TA: Leuk Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Department of Hematology and Stem Cell Unit, IRCCS "Casa Sollievo della Sofferenza" Hospital, San Giovanni Rotondo, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Prognostic implications of leg ulcers from hydroxycarbamide therapy in patients with essential throm...
Next Document: Substituent effects on the structure-property relationship of unsymmetrical methyloxy and methoxycar...