Document Detail


Comparison between the gene expression profile of human Müller cells and two spontaneous Müller cell lines.
MedLine Citation:
PMID:  17962478     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Müller cells are the principal glial cells in the retina. They play important functions in this tissue. Alterations in Müller cell behavior were observed in the retinal tissue of patients with proliferative diabetic retinopathy. The purpose of this study was to determine the gene expression profile of normal human Müller cells (NHMCs) and to compare it with that of two spontaneously generated human Müller cell lines (HMCLs) from type 1 and type 2 diabetic donors by using serial analysis of gene expression (SAGE). METHODS: Approximatively 50,000 tags were sequenced for each SAGE library. Identification of the transcripts was obtained by matching the 15-bp tags with the UniGene and GenBank databases. Classification of the genes was based on the updated information of the genome directory found at the National Center for Biotechnology Information (NCBI) Web site. RESULTS: SAGE was used to characterize the entire transcriptome of human Müller cells and to compare these data with those from Müller cell lines generated from type 1 and type 2 diabetic donors. The transcriptome of NHMCs and HMCLs demonstrated that "metabolism" and "protein synthesis" are the two main categories of genes expressed by human Müller cells. Only 106 genes are differentially expressed between NHMCs and HMCLs. CONCLUSIONS: The SAGE libraries reported in this article provide the gene expression profile of NHMCs and HMLCs. It thus represents a valuable source of information regarding the function of Müller cells as well as their role in the development of diabetic retinopathy.
Authors:
Caroline B Lupien; Carl Bolduc; Solange Landreville; Christian Salesse
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  48     ISSN:  0146-0404     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-26     Completed Date:  2007-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5229-42     Citation Subset:  IM    
Affiliation:
Unité de Recherche en Ophtalmologie, Centre de Recherche du CHUQ, Ste-Foy, Québec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Biological Markers / metabolism
Cell Line
Computational Biology
Diabetes Mellitus, Type 1 / complications*
Diabetes Mellitus, Type 2 / complications*
Diabetic Retinopathy / etiology,  metabolism*
Expressed Sequence Tags
Gene Expression Profiling*
Gene Expression Regulation / physiology*
Humans
Neuroglia / cytology,  metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Biological Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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