Document Detail


Comparison between experimental and theoretical values of effectiveness factor in cephalosporin C production process with immobilized cells.
MedLine Citation:
PMID:  18576015     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Cells ofCephalosporium acretnonium ATCC 48272 immobilized in calcium alginate beads were utilized for cephalosporin C production and the results were compared with those obtained with free cells. The experiments were performed with synthetic medium containing glucose and sucrose as carbon and energy sources. Experimental effectiveness factor values were obtained at various cell and dissolved-oxygen concentrations, considering Monod kinetics for the respiration rate, and were compared with the values calculated with zero-order kinetics in spherical bioparticle. The results showed that the assumption of oxygen limitation by diffusion in the bioparticle was correct, and that cephalosporin C production with immobilized cells is perfectly viable, although a slightly lower rate than that obtained in the free cell process was observed.
Authors:
M L Araujo; R C Giordano; C O Hokka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  70-72     ISSN:  0273-2289     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  1998  
Date Detail:
Created Date:  2008-06-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  493-504     Citation Subset:  -    
Affiliation:
Departamento de Engenharia Química, Universidade Federal de São Carlos, P. O. Box 676, P. Code 13565-905, São Carlos-S.P., Brazil.
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