Document Detail


Comparison between stem cells harvested from wet and dry lipoaspirates.
MedLine Citation:
PMID:  22853627     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adipose-derived stem cells (ASC) are usually isolated from lipoaspirates, but it is not known if the anesthetic solution injected into adipose tissue affects cell yield and functions. Two different samples were drawn from the abdominal region of female subjects. In the first, a physiological solution containing lidocaine/adrenaline was injected (wet liposuction, WL), while in the contralateral area, the sample was collected without injecting any solution (dry liposuction, DL). The aspirates were processed to investigate the yield of the stromal-vascular fraction (SVF) cells and ASC frequency, growth rate, apoptosis, and differentiation potential. The solid dried mass of fresh WL isolates was lower than that of DL isolates (p < 0.01) due to the presence, in the former, of a liquid solution. As a consequence, the amount of WL-SVF cells was 18.7% lower than those obtained from DL (p < 0.01); this difference was also observed under culture conditions. In addition, the number of colony-forming unit-fibroblasts (CFU-Fs) obtained from 1 × 10(3) SVF cells was 25.5% lower in WL-aspirates than DL-aspirates (p < 0.05) owing, at least in part, to the observed presence of ASC [corrected] in the liquid solution of the WL isolates. After WL and DL, no differences were observed in ASC growth rate, apoptosis, or differentiation potential toward adipogenic, osteogenic, and endothelial cell lineages. In conclusion, WL yields about 40% fewer ASC than DL due to the combined effect of tissue dilution and the reduced frequency of ASC in the SVF. The main biological features of ASC are suitable for cell-based therapies.
Authors:
Claudio Muscari; Francesca Bonafè; Emanuela Fiumana; Carlo Maria Oranges; Valentina Pinto; Claudio Marcello Caldarera; Carlo Guarnieri; Paolo Giovanni Morselli
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-09-04
Journal Detail:
Title:  Connective tissue research     Volume:  54     ISSN:  1607-8438     ISO Abbreviation:  Connect. Tissue Res.     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-09     Completed Date:  2013-06-25     Revised Date:  2013-12-23    
Medline Journal Info:
Nlm Unique ID:  0365263     Medline TA:  Connect Tissue Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  34-40     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / cytology,  metabolism
Adipose Tissue / cytology*
Adolescent
Adult
Adult Stem Cells / cytology*
Apoptosis
Cell Count
Cell Differentiation
Cell Proliferation
Cell Survival
Cells, Cultured
Colony-Forming Units Assay
Female
Humans
Lipectomy / methods*
Middle Aged
Multipotent Stem Cells / cytology*
Tissue and Organ Harvesting / methods*
Young Adult
Comments/Corrections
Erratum In:
Connect Tissue Res. 2013;54(6):450

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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