Document Detail


Comparison of the archaeal community in the fermentative compartment and faeces of the cow and the rabbit.
MedLine Citation:
PMID:  20417714     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The archaeal community in the fermentative compartment and faeces of the cow and the rabbit were compared by analysis capillary electrophoresis single-stranded conformation polymorphism (CE-SSCP) profiles of 16S rRNA genes. Ruminal and faecal contents were sampled in five cows for three weeks. Hard and soft faeces were collected in 14 rabbits for three consecutive weeks and caecal contents were sampled in the third week. The archaeal community differed according to the host species (ANOSIM-R=0.53 and 0.72 respectively for the comparison of the fermentative compartments and faeces; P<0.001) and to the location within the digestive tract of both species (ANOSIM-R=0.37, 0.52 respectively for the cow and the rabbit; P<0.001). In both species, the archaeal community of the digestive tract was stable over weeks and varied very little between individual animals. The structure (NS) and the richness index (9.9+/-2.7, 10.1+/-3.1 respectively, NS) of the archaeal community were similar for the caecal content and the soft faeces which permitted to use the latter as a representative indicator.
Authors:
Rory Julien Michelland; Valérie Monteils; Sylvie Combes; Laurent Cauquil; Thierry Gidenne; Laurence Fortun-Lamothe
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2010-04-24
Journal Detail:
Title:  Anaerobe     Volume:  16     ISSN:  1095-8274     ISO Abbreviation:  Anaerobe     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-27     Completed Date:  2010-12-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9505216     Medline TA:  Anaerobe     Country:  England    
Other Details:
Languages:  eng     Pagination:  396-401     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Institut National de la Recherche Agronomique-UMR 1289, Tissus Animaux, Nutrition, Digestion, Ecosystème et Métabolisme, Castanet-Tolosan, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Archaea / classification*,  genetics*
Biodiversity*
Cattle
Cecum / microbiology*
DNA, Archaeal / genetics
DNA, Ribosomal / genetics
Electrophoresis, Capillary
Feces / microbiology*
Polymorphism, Single-Stranded Conformational
RNA, Ribosomal, 16S / genetics
Rabbits
Chemical
Reg. No./Substance:
0/DNA, Archaeal; 0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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