| Comparison of arachidonic acid metabolism between myofibroblasts and fibroblasts isolated from rat palatal mucoperiosteum. | |
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MedLine Citation:
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PMID: 3129015 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Myofibroblasts were cultured successfully from experimental wound tissue in rat palatal mucoperiosteum. Arachidonic acid metabolizing activity in cultured myofibroblasts was compared with that in fibroblasts cultured from normal mucoperiosteum. Prostaglandins biosynthesized from [14C]arachidonic acid in cell-free homogenates of both myofibroblasts and fibroblasts were prostaglandins D2, E2 and F2 alpha, and the activity producing each prostaglandin was not significantly different between the myofibroblasts and the fibroblasts, whereas smooth muscle cells, which are histologically similar to myofibroblasts, produced mainly 6-ketoprostaglandin F1 alpha, and relatively small amounts of prostaglandin E2. The release of arachidonic acid from cells prelabeled with [14C]arachidonic acid was compared among three types of cell. The calcium ionophore A23187 strongly enhanced arachidonic acid release in all three cell types. Bradykinin, 5-hydroxytryptamine and prostaglandin F2 alpha affected the stimulation of arachidonic acid release in the fibroblasts but were less or not effective in the myofibroblasts and smooth muscle cells. In addition, prostaglandin E2 biosynthesized in response to several stimuli was measured by radioimmunoassay. The content of prostaglandin E2 correlated closely with arachidonic acid release. In this study, we showed homogeneity between the myofibroblasts and fibroblasts in prostaglandin synthesizing activity and similarity in response to various stimuli between the myofibroblasts and smooth muscle cells, from the standpoint of arachidonic acid metabolism. |
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Authors:
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Y Baba; I Morita; T Susami; T Kuroda; S Murota |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 960 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1988 May |
Date Detail:
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Created Date: 1988-06-03 Completed Date: 1988-06-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 67-72 Citation Subset: IM |
Affiliation:
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Department of Orthodontics II, Faculty of Dentistry, Tokyo Medical and Dental University, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid Arachidonic Acids / metabolism* Calcimycin / pharmacology Cell-Free System Cells, Cultured Dinoprost Fibroblasts / metabolism* Muscle, Smooth / metabolism* Periosteum / metabolism Prostaglandins / biosynthesis Prostaglandins F / analysis Rats |
| Chemical | |
Reg. No./Substance:
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0/Arachidonic Acids; 0/Prostaglandins; 0/Prostaglandins F; 506-32-1/Arachidonic Acid; 52665-69-7/Calcimycin; 551-11-1/Dinoprost |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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