Document Detail


Comparison of apex and bottom secretion efficiency at chromaffin cells as measured by amperometry.
MedLine Citation:
PMID:  17316959     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In chromaffin cells, the exocytosis of neuromediators involves the fusion between a secretory vesicle and the cell membrane. Many techniques based on electrophysiology, electrochemistry and fluorescence microscopy allow the study of such a complex process at active zones of single immobilized cells. These techniques can provide an effective analysis either at the apex, either at the base of the cell adhering onto a substrate. For instance, patch-clamp (electrophysiology) and amperometry (electrochemistry) deal with detection at the exposed top of the cell, whereas evanescent field microscopy concerns mainly its bottom, i.e., the zone on which the cell rests onto the surface. However, in chromaffin cells, comparison between the two sets of methods remains to be established and whether apex fusion events are comparable or not to those observed at the base of the cell is an open question. In this work, we compare both active zones upon using the same measurement method, viz., by performing electrochemical detection at these both poles (top and bottom) of bovine chromaffin cells. This is performed upon using carbon fiber microelectrodes (apical analysis) and planar ITO transparent (basal analysis) electrodes, respectively. Our results indicate that the processes monitored at each pole differ though the same technique is used.
Authors:
Christian Amatore; Stéphane Arbault; Frédéric Lemaître; Yann Verchier
Related Documents :
9135999 - Genetic analysis of default mating behavior in saccharomyces cerevisiae.
10365439 - Mouse c2 myoblast cells resist hvj (sendai virus)-mediated cell fusion in the prolifera...
16483559 - Ps exposure increases the susceptibility of cells to fusion with dotap liposomes.
6342809 - Establishment of a myoid cell clone from rat thymus.
18562539 - Effects of hemagglutinin-neuraminidase protein mutations on cell-cell fusion mediated b...
10630409 - Overexpression of a dominant-negative type ii tgfbeta receptor tagged with green fluore...
3927579 - Thymoma and thymic carcinoma. relation of thymoma epithelial cells to the cortical and ...
9207859 - Human placenta does not reduce azt (zidovudine) to 3'-amino-3'-deoxythymidine.
23365669 - Arginase treatment prevents the recovery of canine lymphoma and osteosarcoma cells resi...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-01
Journal Detail:
Title:  Biophysical chemistry     Volume:  127     ISSN:  0301-4622     ISO Abbreviation:  Biophys. Chem.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-03-26     Completed Date:  2007-05-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0403171     Medline TA:  Biophys Chem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  165-71     Citation Subset:  IM    
Affiliation:
Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC 8640 PASTEUR, 24 rue Lhomond, 75231 Paris cedex 05. France. Christian.Amatore@ens.fr
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cell Membrane / physiology*
Cells, Cultured
Chromaffin Cells / physiology*
Electrophysiology*
Exocytosis / physiology*
Optics and Photonics

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Common and specific genetic influences on EEG power bands delta, theta, alpha, and beta.
Next Document:  A preliminary process design and economic assessment of a catalyst rejuvenation process for waste di...