Document Detail

Comparison of the antioxidant effects of synovial fluid from equine metacarpophalangeal joints with those of hyaluronic acid and chondroitin sulfate.
MedLine Citation:
PMID:  20405582     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate the antioxidant effects of synovial fluid (SF) pooled from metacarpophalangeal joints of healthy horses and horses with various pathological conditions, and to compare then with the antioxidant effects of hyaluronic acid (HA) and chondroitin sulfate (CS). SAMPLE POPULATION: SF from 1 metacarpophalangeal joint was obtained from 42 horses immediately after humane slaughter. Samples were classified into 3 groups on the basis of origin: healthy joints or joints with chronically damaged cartilage or vascularly congested synovial membranes as detected via macroscopic evaluation. PROCEDURES: Antioxidant effects were evaluated by use if rat liver microsomal fractions treated with Fe(3+)-ascorbate as a free radical generator system leading to oxidative stress. Amounts of thiobarbituric-reactive substances and glutathione transferase (GSH-T) conjugation activity were measured. RESULTS: SF from healthy and chronically damaged joints inhibited microsomal lipid peroxidation, whereas SF from joints with congested synovial membranes had only a slight effect. Hyaluronic acid and CS did not inhibit microsomal lipid peroxidation. Moreover, GSH-T activity was detected in all SF samples as well as HA and CS protected rat microsomal GSH-T activity against oxidative damage. Only SF samples from joints with congested synovial membranes protected microsomal thiols against oxidation, an effect also evident with HA and CS. CONCLUSIONS AND CLINICAL RELEVANCE: The antioxidant mechanisms associated with the response to metacarpophalangeal joint damage in horses appeared to act on different targets, depending on whether the damage was acute or chronic.
Andrea J M?ller; Mar?a E Letelier; Marco A Galleguillos; Alfredo E Molina-Berr?os; H?ctor H Adarmes
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of veterinary research     Volume:  71     ISSN:  0002-9645     ISO Abbreviation:  Am. J. Vet. Res.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-20     Completed Date:  2010-05-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375011     Medline TA:  Am J Vet Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  399-404     Citation Subset:  IM    
Department of Animal Biological Sciences, School of Veterinary and Animal Sciences, Universidad de Chile, La Pintana, Santiago, Chile.
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MeSH Terms
Antioxidants / metabolism,  pharmacology
Chondroitin Sulfates / pharmacology*
Glutathione Transferase / metabolism
Horses / physiology*
Hyaluronic Acid / pharmacology*
Lipid Peroxidation / drug effects
Microsomes, Liver / metabolism*
Rats, Sprague-Dawley
Synovial Fluid / physiology*
Reg. No./Substance:
0/Antioxidants; 9004-61-9/Hyaluronic Acid; 9007-28-7/Chondroitin Sulfates; EC Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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