Document Detail


Comparison of anticancer activity between lactoferrin nanoliposome and lactoferrin in Caco-2 cells in vitro.
MedLine Citation:
PMID:  23743119     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The anticancer activities of Lactoferrin (Lf) and Lf nanoliposomes in Caco-2 cells were observed in this study, and mitochondrial function (MTT assay), count kit-8(CCK-8), detection of intracellular reactive oxygen species (ROS) and apoptosis induction (AO/EB staining) assays were used to evaluate the anticancer activity. MTT results demonstrated that Lf nanoliposomes and Lf reduced the mitochondrial activity of cells in a manner of dose and time effect, and the viabilities of Caco-2 cell were significantly decreased in vitro following exposure to Lf nanoliposomes at the concentrations of 5 and 10 mg/mL. LDH leakage and ROS significantly increased in cells exposed to Lf nanoliposomes(⩾5mg/mL), while Lf induced ROS only at higher doses (10mg/mL). CCK-8 evaluation of cell proliferation and AO/EB double staining supported the anti-proliferative effects of Lf liposomes. Our findings demonstrated that the presence of Lf nanoliposome is more significant than Lf in inhibiting human tumor cells proliferation. Therefore, it can be concluded that Lf nanoliposomes are a potential therapeutic modality in the management of tumors.
Authors:
Jieqing Ma; Rongfa Guan; Haitao Shen; Fei Lu; Chaogeng Xiao; Mingqi Liu; Tianshu Kang
Related Documents :
22991499 - Interferon-gamma-induced nitric oxide inhibits the proliferation of murine renal cell c...
24704589 - Protective effect of acetyl-l-carnitine on propofol-induced toxicity in embryonic neura...
24651449 - Differences between the cell populations from the peritenon and the tendon core with re...
24500019 - Mitotic spindle and mitotic cell volumes in the root meristem of zea mays.
25007959 - Exosomal mir-221/222 enhances tamoxifen resistance in recipient er-positive breast canc...
24920049 - Dynamic mitochondrial changes during differentiation of p19 embryonic carcinoma cells i...
11732159 - Anchorage-dependent chick embryo fibroblasts synthesise dna and proliferate when cultur...
22396589 - Antiproliferative small-molecule inhibitors of transcription factor lsf reveal oncogene...
17010659 - Polarized growth: maintaining focus on the tip.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-6-3
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  -     ISSN:  1873-6351     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-6-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013. Published by Elsevier Ltd.
Affiliation:
Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, China Jiliang University, Hangzhou 310018, P. R. China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Complement activation in diseases presenting with thrombotic microangiopathy.
Next Document:  Effects of Korean red ginseng extract on acute renal failure induced by gentamicin and pharmacokinet...