| Comparison of antibody responses to hen's egg and cow's milk proteins in orally sensitized rats and food-allergic patients. | |
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MedLine Citation:
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PMID: 10753016 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: No adequate enteral sensitization models are available to study food allergy and the allergenicity of food proteins. To further validate an enteral brown Norway (BN) rat sensitization model under development, we studied specific protein recognition to determine whether a comparable pattern of proteins is recognized by the rat immune system and the human immune system. METHODS: The animals were exposed to either ovalbumin as a positive reference control, hen's egg-white-protein extract, or a cow's milk preparation by daily gavage dosing (0.5, 1, 2.5, 5, 10, or 15 mg protein per rat/day) for 9 weeks. No adjuvants were used during the sensitization studies. The specificities of antibodies against hen's egg-white proteins or cow's-milk proteins in sera from orally sensitized rats and food-allergic patients were studied and compared by immunoblotting. RESULTS: The IgG and IgE antibodies to hen's egg-white proteins and cow's-milk proteins present in sera from orally sensitized rats and food-allergic patients showed a comparable pattern of protein recognition. CONCLUSIONS: Upon daily intragastric exposure to food allergens, the specificities of the induced antibody responses in the BN rat resemble those found in food-allergic patients. These studies add further support to the hypothesis that the BN rat may provide a suitable animal model for food allergy research and research on the allergenicity of food proteins. |
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Authors:
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L M Knippels; H P van der Kleij; S J Koppelman; G F Houben; A H Penninks |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Allergy Volume: 55 ISSN: 0105-4538 ISO Abbreviation: Allergy Publication Date: 2000 Mar |
Date Detail:
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Created Date: 2000-04-27 Completed Date: 2000-04-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7804028 Medline TA: Allergy Country: DENMARK |
Other Details:
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Languages: eng Pagination: 251-8 Citation Subset: IM |
Affiliation:
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TNO Nutrition and Food Research Institute, Zeist, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Allergens
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immunology* Animals Antibody Specificity / immunology Blotting, Western Child Child, Preschool Dietary Proteins / immunology Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Food Hypersensitivity / immunology Humans Immune System Immunoglobulin E / analysis* Infant Male Milk / adverse effects Milk Hypersensitivity / etiology, immunology* Milk Proteins / immunology* Ovalbumin / immunology* Passive Cutaneous Anaphylaxis Rats Rats, Inbred BN |
| Chemical | |
Reg. No./Substance:
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0/Allergens; 0/Dietary Proteins; 0/Milk Proteins; 37341-29-0/Immunoglobulin E; 9006-59-1/Ovalbumin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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