| Comparison of anterior-posterior development in the porcine versus chicken embryo, using goosecoid expression as a marker. | |
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MedLine Citation:
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PMID: 11720135 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During early embryonic development, pig and chicken embryos share striking morphological similarities. In the present study, the timing and location of expression of mRNA for goosecoid (gsc), a gene classically expressed in the nodal region of developing embryos, was examined and compared in preprimitive streak and gastrulating pig and chicken embryos. The expression of gsc appeared first in the hypoblast and second in the hypoblast of pig and chicken embryos. Because gsc expression in these tissues was not symmetrical, gsc appears to be a useful marker for the onset of embryonic polarity. During gastrulation in both species, gsc expression became confined to cells in and around the node, in the epiblast and mesoderm layers. The only significant species-related difference in the distribution of gsc expression at these stages of development was the presence of gsc expression in the gut endoderm of chicken but not pig embryos. Certainly, our results suggest that the molecular mechanisms that control anterior-posterior development in different classes of vertebrates are remarkably similar. In addition, we were able to demonstrate that the pattern of gsc expression appears to provide a more sensitive and accurate means of determining the developmental stage of early porcine embryos than the more commonly used trophoblast or embryoblast size. Using gsc expression and accompanying embryo morphometric changes, we were able to develop a four-point scale that may offer a more accurate means of quantifying early embryo development in pigs. |
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Authors:
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S A van de Pavert; H Schipper; A A de Wit; N M Soede; R van den Hurk; M A Taverne; M L Boerjan; H W Stroband |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Reproduction, fertility, and development Volume: 13 ISSN: 1031-3613 ISO Abbreviation: Reprod. Fertil. Dev. Publication Date: 2001 |
Date Detail:
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Created Date: 2001-11-26 Completed Date: 2001-12-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8907465 Medline TA: Reprod Fertil Dev Country: Australia |
Other Details:
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Languages: eng Pagination: 177-85 Citation Subset: IM |
Affiliation:
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Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers Chick Embryo / growth & development* Embryonic and Fetal Development* Gastrula / physiology Gene Expression* Goosecoid Protein Homeodomain Proteins / genetics* In Situ Hybridization RNA, Messenger / analysis Repressor Proteins* Species Specificity Swine / embryology* Time Factors Tissue Distribution Transcription Factors* |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Goosecoid Protein; 0/Homeodomain Proteins; 0/RNA, Messenger; 0/Repressor Proteins; 0/Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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