| Comparison of the analysis of beta-blockers by different techniques. | |
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MedLine Citation:
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PMID: 19879818 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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In this work we have compared three analytical techniques (ELISA, GC-MS, and LC-MS) for the analysis of 16 beta-blockers: acebutolol, alprenolol, atenolol, betaxolol, bisoprolol, carteolol, labetalol, metipranolol, metoprolol, nadolol, oxprenolol, pindolol, propranolol, sotalol, timolol, and bupranolol. Several sample-preparation methods were optimized for each technique and enabled compounds of interest to be extracted from small urine samples (1-2.5 mL). The results enabled us to assess the possibilities and the sensitivity of each technique for application to doping tests. ELISA, whose selectivity is very poor and sensitivity the lowest one, is, nevertheless, useful as a rapid screening method. GC/MS and LC/MS provide confirmation procedures with the identification and quantification of the beta-blockers with good sensitivity, accuracy, precision. The LC-MS analytical procedure allows the determination of the target analytes in the lower ng/mL range (0.53-2.23 ng/mL). The methodology was applied to the analysis of beta-blockers in different urines. |
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Authors:
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E Pujos; C Cren-Olivé; O Paisse; M M Flament-Waton; M F Grenier-Loustalot |
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Publication Detail:
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Type: Journal Article Date: 2009-10-17 |
Journal Detail:
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Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 877 ISSN: 1873-376X ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-11-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 4007-14 Citation Subset: IM |
Affiliation:
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Service Central d'Analyse, USR 059, CNRS, 69360 Solaize, France. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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