Document Detail


Comparison of the activities of CaMV 35S and FMV 34S promoter derivatives in Catharanthus roseus cells transiently and stably transformed by particle bombardment.
MedLine Citation:
PMID:  9106518     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Activities of several CaMV 35S and FMV 34S promoter derivatives fused to the gusA reporter gene were compared in suspension-cultured Catharanthus roseus cells that were transiently and stably transformed using particle bombardment. Our data demonstrate that the 35S and a deletion derivative of the 34S promoter combined with particle bombardment form useful tools for genetic engineering of C. roseus cells. Our results disagree on several points with activities of 35S and 34S promoter derivatives reported for tobacco, indicating that absolute and relative promoter activities can differ between plant species.
Authors:
L van der Fits; J Memelink
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant molecular biology     Volume:  33     ISSN:  0167-4412     ISO Abbreviation:  Plant Mol. Biol.     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-05-12     Completed Date:  1997-05-12     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9106343     Medline TA:  Plant Mol Biol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  943-6     Citation Subset:  IM    
Affiliation:
Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, the Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Alkaloids / biosynthesis
Caulimovirus / genetics
Cells, Cultured
Genes, Reporter
Genetic Engineering / methods
Genetic Techniques*
Glucuronidase / genetics
Mosaic Viruses / genetics*
Plants, Medicinal / genetics*
Promoter Regions, Genetic / genetics*
Transformation, Genetic*
Chemical
Reg. No./Substance:
0/Alkaloids; EC 3.2.1.31/Glucuronidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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