Document Detail


Comparison of Yarrowia lipolytica and Pichia pastoris Cellular Response to Different Agents of Oxidative Stress.
MedLine Citation:
PMID:  23546869     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Yeast cells exposed to adverse conditions employ a number of defense mechanisms in order to respond effectively to the stress effects of reactive oxygen species. In this work, the cellular response of Yarrowia lipolytica and Pichia pastoris to the exposure to the ROS-inducing agents' paraquat, hydrogen peroxide, and increased air pressure was analyzed. Yeast cells at exponential phase were exposed for 3 h to 1 mM paraquat, to 50 mM H2O2, or to increased air pressure of 3 or 5 bar. For both strains, the cellular viability loss and lipid peroxidation was lower for the cells exposed to increased air pressure than for those exposed to chemical oxidants. The glutathione induction occurred only in Y. lipolytica strain and reached the highest level as a response to PQ exposure. In general, antioxidant enzymes were more expressed in Y. lipolytica than in P. pastoris. The enzyme superoxide dismutase was induced in both strains under all the oxidant conditions but was dependent on the cellular growth phase, being undetectable in non-growing cells, whereas glutathione reductase was more induced in those conditions. Hydrogen peroxide was the most efficient inducer of catalase. Both yeast cultures underwent no cellular growth inhibition with increased air pressure, indicating that these yeast species were able to adapt to the oxidative stressful environment.
Authors:
Marlene Lopes; Manuel Mota; Isabel Belo
Related Documents :
6273179 - Use of labetalol in hypertensive patients during discontinuation of clonidine therapy.
21807549 - Waveform descriptor for pulse onset detection of intracranial pressure signal.
21131159 - How much is residents' distress detection performance during a clinical round related t...
6462119 - Effect of chronic administration of clonidine and propranolol on the myocardium of spon...
2897989 - Desensitization of vascular and platelet alpha 2-adrenoceptors in rabbits with perineph...
17995999 - Reduced blood pressure lability during emergence from anaesthesia in rats: a pilot stud...
2144489 - Prevalence and determinants of left ventricular diastolic filling abnormalities in an u...
8756269 - On the pressure and flow-rate distributions in tree-like and arterial-venous networks.
15506599 - Intraocular pressure variability in the anesthetized rat: a spectral analysis.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-4-2
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  -     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-4-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal, marlenelopes@deb.uminho.pt.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Process Characterization of hCTLA4Ig Production in Transgenic Rice Cell Cultures Using a 3-L Bioreac...
Next Document:  Role of Met93 and Thr96 in the Lid Hinge Region of Rhizopus chinensis Lipase.