Document Detail


Comparison of transfection efficiency of nonviral gene transfer reagents.
MedLine Citation:
PMID:  20585901     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study compared six commercially available reagents (Arrest-In, ExpressFect, FuGENE HD, jetPEI, Lipofectamine 2000, and SuperFect) for gene transfection. We examined the efficiency and cytotoxicity using nine different cell lines (MC3T3-E1 mouse preosteoblasts, PT-30 human epithelial precancer cells, C3H10T1/2 mouse stem cells, MCF-7 human breast cancer cells, HeLa human cervical cancer, C2C12 mouse myoblasts, Hep G2 human hepatocellular carcinoma, 4T1 mouse mammary carcinoma, and HCT116 human colorectal carcinoma), and primary cells (HEKn human epidermal keratinocytes) with two different plasmid DNAs encoding luciferase or β-galactosidase in the presence or absence of serum. Maximal transfection efficiency in MC3T3-E1, C3H10T1/2, HeLa, C2C12, Hep G2, and HCT116 was seen using FuGENE HD, in PT-30, 4T1, and HEKn was seen using Arrest-In, and in MCF-7 was seen using jetPEI. Determination of cytotoxicity showed that the largest amount of viable cells was found after transfection with jetPEI and ExpressFect. These results suggest that FuGENE HD is the most preferred transfection reagent for many cell lines, followed by Arrest-In and jetPEI. These results may be useful for improving nonviral gene and cell therapy applications.
Authors:
Seiichi Yamano; Jisen Dai; Amr M Moursi
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Molecular biotechnology     Volume:  46     ISSN:  1559-0305     ISO Abbreviation:  Mol. Biotechnol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-09-29     Completed Date:  2011-02-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423533     Medline TA:  Mol Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  287-300     Citation Subset:  IM    
Affiliation:
Department of Prosthodontics, New York University College of Dentistry, 345 East 24th Street, 4W, New York, NY 10010, USA. sy23@nyu.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
DNA / genetics
Gene Transfer Techniques*
Humans
Indicators and Reagents*
Luciferases / genetics
Mice
Transfection*
Transgenes
beta-Galactosidase / genetics
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 9007-49-2/DNA; EC 1.13.12.-/Luciferases; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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