Document Detail

A Comparison of Trabecular Meshwork Sphingolipids and Ceramides of Ocular Normotensive and Hypertensive States of DBA/2J Mice.
MedLine Citation:
PMID:  24320088     Owner:  NLM     Status:  Publisher    
Abstract Purpose: To determine the differential profiles of sphingomyelin, sphingoid base, sphingoid base-1-phosphate, and ceramide and their quantitative differences between trabecular meshwork (TM) derived from normotensive and hypertensive intraocular pressure states of DBA/2J mice. Methods: Normotensive and hypertensive state TM were collected from mice and analyzed. Lipid extraction was performed using the Bligh and Dyer method, and the protein concentrations were determined using the Bradford method. The lipids were identified and quantified using appropriate standards with a TSQ Quantum Access Max triple quadrupole mass spectrometer applying class-specific lipid identification settings. Results: The comparative profiles of sphingomyelin, sphingoid base, sphingoid base-1-phosphate, and ceramide between normotensive and hypertensive TM showed several species unique to a phase and as well common between states. Conclusion: The presence or absence of several sphingolipids and ceramides in the normotensive or hypertensive states may contribute to better understanding of the glaucomas.
Yenifer Guerra; Ayman J Aljohani; Genea Edwards; Sanjoy K Bhattacharya
Related Documents :
19429528 - Physiological response to increasing levels of neurally adjusted ventilatory assist (na...
16129818 - Slow breathing improves arterial baroreflex sensitivity and decreases blood pressure in...
2095838 - Coordination of sucking, swallowing and breathing in the newborn: its relationship to i...
3904528 - Inspiratory work with and without continuous positive airway pressure in patients with ...
8693988 - Adaptive changes of autoregulation in chronic cerebral hypotension with arteriovenous m...
18997528 - Using ventilation-induced plethysmographic variations to optimize patient fluid status.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-12-9
Journal Detail:
Title:  Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics     Volume:  -     ISSN:  1557-7732     ISO Abbreviation:  J Ocul Pharmacol Ther     Publication Date:  2013 Dec 
Date Detail:
Created Date:  2013-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9511091     Medline TA:  J Ocul Pharmacol Ther     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine , Miami, Florida.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Control of Outflow Resistance by Soluble Adenylyl Cyclase.
Next Document:  Critical appraisal of evidence supporting the placement of dental implants in patients with neurodeg...