Document Detail

Comparison of ThinPrep and conventional preparations on fine needle aspiration cytology material.
MedLine Citation:
PMID:  10667159     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To compare the various cytologic features on ThinPrep 2000 (TP) (Cytyc Corporation, Marlborough, Massachusetts, U.S.A.) and conventional preparation (CP) specimens from fine needle aspiration cytology (FNAC) material by a semiquantitative scoring system. STUDY DESIGN: In this prospective study a total of 71 consecutive cases were included. In each case, two passes were performed. The first pass was used for conventional preparations, with direct smears made and fixed immediately in 95% alcohol for Papanicolaou stain. For TP preparation a second pass produced material for processing in the ThinPrep 2000. The TP and CP slides were studied independently by two observers and representative slides of CP and TP compared for cellularity, background blood and necrotic cell debris, cell architecture, informative background, presence of monolayer cells, and nuclear and cytoplasmic details by a semiquantitative scoring system. Statistical analysis was performed by Wilcoxon's signed rank test on an SPSS program (Chicago, Illinois, U.S.A.). RESULTS: TP preparations contained adequate diagnostic cells in all cases and were tangibly superior to CP preparations concerning monolayer cells, absence of blood and necrosis, and preservation of nuclear and cytoplasmic detail (statistically significant, Wilcoxon's signed rank test, P < .000). CONCLUSION: TP preparations are superior to conventional preparations with regard to clear background, monolayer cell preparation and cell preservation. It is easier and less time consuming to screen and interpret TP preparations because the cells are limited to smaller areas on clear backgrounds, with excellent cellular preservation. However, TP preparations are more expensive than CP and require some experience for interpretation.
P Dey; U K Luthra; J George; F Zuhairy; S S George; B I Haji
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Acta cytologica     Volume:  44     ISSN:  0001-5547     ISO Abbreviation:  Acta Cytol.     Publication Date:    2000 Jan-Feb
Date Detail:
Created Date:  2000-02-24     Completed Date:  2000-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370307     Medline TA:  Acta Cytol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  46-50     Citation Subset:  IM    
Department of Cytology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
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MeSH Terms
Biopsy, Needle / methods*
Breast Neoplasms / pathology*
Lymph Nodes / pathology*
Lymphatic Metastasis
Prospective Studies
Soft Tissue Neoplasms / pathology*
Thyroid Neoplasms / pathology*

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