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Comparison of Synthetic Oviductal Fluid and G1/G2 Medium under Low-1 Oxygen Atmosphere on Embryo Production and Pregnancy Rates in Nelore (Bos indicus) Cattle.
MedLine Citation:
PMID:  22448771     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In this work, we evaluated whether embryo development and pregnancy rates would be affected by culturing bovine Bos indicus embryos in Synthetic Oviductal Fluid with amino acids (SOFaa) or G1/G2 sequential medium under a low-oxygen atmosphere. Using Ovum Pick Up, we obtained 1,538 oocytes, divided into G1/G2 (n = 783) and SOFaa (n = 755). No difference was observed for blastocyst development among the groups (27.8% ± 14.6 and 34.9% ± 20.0 for G1/G2 and SOFaa respectively, p > 0.05). Transferring the embryos (n = 450) from both groups to recipients resulted in similar pregnancy rates for the G1/G2 (38.4% n = 78/203) compared to the SOFaa (39.7% n = 98/247). Our findings confirm that Bos indicus embryos cultured in SOFaa and G1/G2 under low-oxygen atmosphere have similar in vitro (blastocyst rate) and in vivo (pregnancy rate) developmental capacity. However, embryos cultured in G1/G2 medium have higher cleavage than those cultured in SOFaa medium.
Authors:
Bv Sanches; Jhf Pontes; Ac Basso; Cr Ferreira; F Perecin; Mm Seneda
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-27
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  -     ISSN:  1439-0531     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Blackwell Verlag GmbH.
Affiliation:
In Vitro Brasil Ltda Research Division, Mogi Mirim, SP, Brazil Animal Reproduction Laboratory, DCV-CCA, State University of Londrina - UEL, Londrina, PR, Brazil Faculty of Animal Sciences and Food Engineering - FZEA, University of São Paulo - USP, Pirassununga, SP, Brazil.
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