| Comparison of SpectraCell RA typing and multilocus sequence typing for extended-spectrum-β-lactamase-producing Escherichia coli. | |
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MedLine Citation:
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PMID: 23035198 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Multilocus sequence typing (MLST) is one of the most reliable methods for typing of Escherichia coli, including extended-spectrum-β-lactamase-producing E. coli (ESBL-EC). We investigated the performance of a new typing method, SpectraCell RA (River Diagnostics, Madison, WI), in comparison on MLST on a well-defined collection of ESBL-EC isolates obtained from chicken meat and humans. Ninety-two ESBL-EC isolates obtained from meat and 59 ESBL-EC isolates obtained from human rectal swabs and clinical blood cultures were typed using MLST and SpectraCell RA. The sensitivity and specificity of SpectraCell RA were calculated, using MLST as a reference method. Subsequently, the results of SpectraCell RA were used to determine the relatedness of ESBL-EC isolates from chicken and humans. Using MLST as the gold standard, the performance of SpectraCell RA was evaluated for 3 different cutoff values: 0.99975, 0.99955, and 0.99935. Depending on the cutoff value, the sensitivity was mediocre to unacceptably low, with values of 9.4%, 43.9%, and 66.7%, respectively. When sensitivity increased, the specificity decreased rapidly, from 95.6% to 69.8% and 34.4%, respectively. Also, the number of clusters containing both human and meat samples varied from 0 (0.0%) to 14 (38.9%). Our study shows that SpectraCell RA is not a suitable typing method for ESBL-EC when evaluating relationships of ESBL-EC at the population level. |
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Authors:
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I T M A Overdevest; M Heck; K van der Zwaluw; I Willemsen; J van de Ven; C Verhulst; J A J W Kluytmans |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article Date: 2012-10-03 |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 50 ISSN: 1098-660X ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-11-19 Completed Date: 2013-04-17 Revised Date: 2013-06-05 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 3999-4001 Citation Subset: IM |
Affiliation:
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Department of Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands. i.overdevest@elisabeth.nl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chickens Escherichia coli / classification*, enzymology, genetics*, isolation & purification Escherichia coli Infections / microbiology Humans Meat / microbiology Molecular Epidemiology / methods Molecular Typing / methods* Sensitivity and Specificity beta-Lactamases / secretion* |
| Chemical | |
Reg. No./Substance:
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EC 3.5.2.6/beta-Lactamases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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