Document Detail


Comparison of SpectraCell RA typing and multilocus sequence typing for extended-spectrum-β-lactamase-producing Escherichia coli.
MedLine Citation:
PMID:  23035198     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Multilocus sequence typing (MLST) is one of the most reliable methods for typing of Escherichia coli, including extended-spectrum-β-lactamase-producing E. coli (ESBL-EC). We investigated the performance of a new typing method, SpectraCell RA (River Diagnostics, Madison, WI), in comparison on MLST on a well-defined collection of ESBL-EC isolates obtained from chicken meat and humans. Ninety-two ESBL-EC isolates obtained from meat and 59 ESBL-EC isolates obtained from human rectal swabs and clinical blood cultures were typed using MLST and SpectraCell RA. The sensitivity and specificity of SpectraCell RA were calculated, using MLST as a reference method. Subsequently, the results of SpectraCell RA were used to determine the relatedness of ESBL-EC isolates from chicken and humans. Using MLST as the gold standard, the performance of SpectraCell RA was evaluated for 3 different cutoff values: 0.99975, 0.99955, and 0.99935. Depending on the cutoff value, the sensitivity was mediocre to unacceptably low, with values of 9.4%, 43.9%, and 66.7%, respectively. When sensitivity increased, the specificity decreased rapidly, from 95.6% to 69.8% and 34.4%, respectively. Also, the number of clusters containing both human and meat samples varied from 0 (0.0%) to 14 (38.9%). Our study shows that SpectraCell RA is not a suitable typing method for ESBL-EC when evaluating relationships of ESBL-EC at the population level.
Authors:
I T M A Overdevest; M Heck; K van der Zwaluw; I Willemsen; J van de Ven; C Verhulst; J A J W Kluytmans
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article     Date:  2012-10-03
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  50     ISSN:  1098-660X     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-19     Completed Date:  2013-04-17     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3999-4001     Citation Subset:  IM    
Affiliation:
Department of Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands. i.overdevest@elisabeth.nl
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MeSH Terms
Descriptor/Qualifier:
Animals
Chickens
Escherichia coli / classification*,  enzymology,  genetics*,  isolation & purification
Escherichia coli Infections / microbiology
Humans
Meat / microbiology
Molecular Epidemiology / methods
Molecular Typing / methods*
Sensitivity and Specificity
beta-Lactamases / secretion*
Chemical
Reg. No./Substance:
EC 3.5.2.6/beta-Lactamases
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