Document Detail


Comparison of Southern blot analysis with isotopic and nonisotopic in situ hybridization for the detection of human papillomavirus sequences in invasive carcinoma of the uterine cervix.
MedLine Citation:
PMID:  1315439     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To compare the efficiency of hybridization methods for the detection of HPV genome, 22 cases of invasive squamous cell carcinoma of the uterine cervix were analyzed by Southern blot analysis and in situ hybridization carried out with 35S- and biotin-labeled probes. These cases contained from less than one to as many as 50 copies per cell of HPV 16 and 18 types. To increase the sensitivity of biotinylated probes, a silver enhancement procedure of the peroxidase reaction product was applied. Results showed that in situ hybridization performed with isotopic probes is as sensitive as Southern blot analysis and is more sensitive than that performed with biotin-labeled probe. However, the application of the silver enhancement procedure increases the percentage of HPV-positive cases from 27 to 50%.
Authors:
L D'Amato; S Pilotti; A Rotola; D Di Luca; E Cassai; F Rilke
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc     Volume:  5     ISSN:  0893-3952     ISO Abbreviation:  Mod. Pathol.     Publication Date:  1992 Mar 
Date Detail:
Created Date:  1992-06-03     Completed Date:  1992-06-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8806605     Medline TA:  Mod Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  207-11     Citation Subset:  IM    
Affiliation:
Division of Anatomical Pathology and Cytology, Istituto Nazionale Tumori, Milano, Italy.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Blotting, Southern*
Carcinoma / genetics*,  pathology
DNA, Neoplasm / analysis
DNA, Viral / analysis*
Female
Humans
Neoplasm Invasiveness
Nucleic Acid Hybridization*
Papillomaviridae / genetics*
Sulfur Radioisotopes / diagnostic use
Uterine Cervical Neoplasms / genetics*,  pathology
Chemical
Reg. No./Substance:
0/DNA, Neoplasm; 0/DNA, Viral; 0/Sulfur Radioisotopes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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