Document Detail


A comparison of the sensitivity, specificity, and molecular weight accuracy of three different commercially available Hyaluronan ELISA-like assays.
MedLine Citation:
PMID:  20864567     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Hyaluronan (HA) is a glycosaminoglycan found in the extracellular matrix and ranges from several thousand to millions of daltons in size. HA has importance in various pathological conditions and is known to be elevated in several diseases. Three commonly used, commercially available HA enzyme-linked immunosorbent assay (ELISA)-like assays (from Corgenix, Echelon and R&D) were compared on the basis of accuracy, sample variability and ability to measure a range of HA sizes. The Corgenix HA ELISA-like assay displayed the lowest intra-assay variability [coefficient of variation (CV) = 11.7 ± 3.6%], followed by R&D (CV = 12.3 ± 4.6%) and Echelon (CV = 18.9 ± 9.2%). Interassay variability was also lowest for the Corgenix assay (CV = 6.0%), intermediate for the Echelon assay (9.5%) and highest for the R&D assay (CV = 34.1%). The high interassay variability seen for the R&D assay may have been due to the effect of dilution, since the dilution-independent interassay variability was 15.5%. The concentration of the standard HA was overestimated by the Echelon assay by 85% and underestimated by the R&D and Corgenix assays by 34 and 32%, respectively. The Echelon HA ELISA-like assay was the most effective at measuring all sizes of HA tested (2 MDa and 132, 66 and 6.4 kDa), whereas the Corgenix and R&D assays were unable to detect 6.4 kDa HA. These findings suggest that the Echelon HA ELISA-like assay is better suited for size-sensitive HA measurements but has a relatively high variability. The Corgenix and R&D HA ELISA-like assays have low variability and high accuracy but are not suitable for detecting low-molecular-weight HA.
Authors:
Sarah Haserodt; Metin Aytekin; Raed A Dweik
Related Documents :
10321577 - A high throughput colorimetric cell proliferation assay for the identification of human...
24430157 - Photocontrol of gibberellin levels as related to the unrolling of etiolated wheat leaves.
3674377 - Elimination of coash interference from acetyl-coa cycling assay by maleic anhydride.
23839327 - Analytical determination of specific 4,4'-methylene diphenyl diisocyanate hemoglobin ad...
18695957 - Detection of salmonella species in foodstuffs.
9134667 - An immunoassay for teq screening of dioxin/furan samples: current status of assay and a...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-23
Journal Detail:
Title:  Glycobiology     Volume:  21     ISSN:  1460-2423     ISO Abbreviation:  Glycobiology     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2010-12-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9104124     Medline TA:  Glycobiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  175-83     Citation Subset:  IM    
Affiliation:
Department of Pathobiology/Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Inhalation heterogeneity from subresidual volumes in elite divers.
Next Document:  Galectin multimerization and lattice formation are regulated by linker region structure.