Document Detail

Comparison of Polymerase Chain Reaction with Bacterial 16s Primers to Blood Culture to Identify Bacteremia in Dogs with Suspected Bacterial Endocarditis.
MedLine Citation:
PMID:  21689155     Owner:  NLM     Status:  Publisher    
Background: Identification of the bacterial organism in dogs with endocarditis is challenging. Human studies have reported the utility of the polymerase chain reaction (PCR) to amplify and identify bacterial nucleic acid from infected valvular tissue and blood. Hypothesis/Objectives: We hypothesized that PCR using primers designed to amplify the bacterial 16s gene would identify circulating bacteria in dogs with suspected bacterial endocarditis more consistently than standard blood culture techniques. Animals: Eighteen dogs with suspected bacterial endocarditis based upon clinical and echocardiographic findings. Fifteen clinically normal dogs served as negative controls. Methods: Prospective study of dogs evaluated for suspect endocarditis at 6 veterinary hospitals. A blood sample was drawn from all dogs and evaluated with both a single-sample PCR and standard 3-sample blood culture techniques. Results: Blood culture identified noncontaminant bacteria in 6/18 study animals (33%) and 1 control dog; PCR identified noncontaminant bacteria in 7/18 study animals (39%). There were no study animals in which the 2 tests identified different bacteria (κ= 1.0). However, bacteria were identified by both techniques in only 2/18 study animals. When results from both PCR and blood culture were considered together, a noncontaminant bacterial organism was identified in 11/18 study animals (61%). Conclusion and Clinical Importance: The results of this study suggest that although single sample PCR with 16s primers was not more sensitive than blood culture for detection of bacteremia in dogs with suspect endocarditis, performing both techniques simultaneously did increase the likelihood of identification of bacteria in blood.
K M Meurs; A M Heaney; C E Atkins; T C Defrancesco; P R Fox; B W Keene; H B Kellihan; M W Miller; M A Oyama; J L Oaks
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-6-20
Journal Detail:
Title:  Journal of veterinary internal medicine / American College of Veterinary Internal Medicine     Volume:  -     ISSN:  1939-1676     ISO Abbreviation:  -     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-6-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8708660     Medline TA:  J Vet Intern Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 by the American College of Veterinary Internal Medicine.
Department of Veterinary Clinical Sciences, Washington State University College of Veterinary Medicine, Pullman, WA Departments of Veterinary Microbiology and Pathology, Washington State University College of Veterinary Medicine, Pullman, WA Department of Clinical Sciences, North Carolina State College of Veterinary Medicine, Raleigh, NC Caspary Institute, The Animal Medical Center, New York, NY Department of Medical Sciences, University of Wisconsin School of Veterinary Medicine, Madison, WI Department of Small Animal Clinical Sciences, Texas A&M College of Veterinary Medicine, College Station, TX Department of Clinical Studies, School of Veterinary Medicine University of Pennsylvania, Philadelphia, PA.
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