Document Detail


Comparison of PhoP binding to the tuaA promoter with PhoP binding to other Pho-regulon promoters establishes a Bacillus subtilis Pho core binding site.
MedLine Citation:
PMID:  9611818     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The phosphate-deficiency response in Bacillus subtilis is regulated by PhoP and PhoR, a pair of two-component regulatory proteins. PhoR is a histidine kinase and PhoP is a response regulator. Genetic evidence indicates that the Pho-regulon genes, which are induced or repressed under phosphate starvation conditions, are regulated by PhoP and PhoR at the transcriptional level. It has previously been shown that PhoP binds to four Pho-regulon promoters in both unphosphorylated and phosphorylated forms. This study demonstrates that another Pho-regulon gene promoter, the tuaA promoter preceding the operon which is responsible for cell wall teichuronic acid synthesis, is also transcriptionally regulated and is bound by PhoP. The binding affinity for phosphorylated PhoP was about 10-fold higher than that for unphosphorylated PhoP. Both unphosphorylated and phosphorylated PhoP bound upstream of the -20 region in the tuaA promoter. By aligning the PhoP-binding sites within the Pho-regulon promoters, a consensus core PhoP-binding region composed of four TT(A/T)ACA direct repeats, each separated by 5 +/- 2 non-conserved nucleotides was identified. PhoP, phosphorylated or unphosphorylated, binds to such a sequence in all Pho-regulon promoters studied. Phosphorylated PhoP binds to the core binding region with high affinity and to additional regions surrounding this region with similar or lower affinity.
Authors:
W Liu; F M Hulett
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  144 ( Pt 5)     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-07-07     Completed Date:  1998-07-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1443-50     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, University of Illinois, Chicago 60607, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacillus subtilis / enzymology,  genetics*,  growth & development,  metabolism
Bacterial Proteins / metabolism*
Base Sequence
Binding Sites
Consensus Sequence
DNA Footprinting
DNA-Binding Proteins
Gene Expression Regulation, Bacterial
Genes, Bacterial
Genes, Regulator
Molecular Sequence Data
Phosphates / metabolism*
Promoter Regions, Genetic*
Protein Kinases / genetics*,  metabolism*
Regulon*
Sequence Analysis, DNA
Transcription Factors / metabolism
Uronic Acids / metabolism
beta-Galactosidase / analysis
Grant Support
ID/Acronym/Agency:
GM33471/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA-Binding Proteins; 0/Phosphates; 0/Transcription Factors; 0/Uronic Acids; 125360-99-8/PhoP protein, Bacteria; 37251-79-9/teichuronic acid; EC 2.7.-/Protein Kinases; EC 2.7.3.-/protein-histidine kinase; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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