Document Detail


Comparison of a PCR assay in whole blood and serum specimens for canine brucellosis diagnosis.
MedLine Citation:
PMID:  20643887     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The performance of a serum PCR assay was compared with that of a blood PCR assay for the diagnosis of canine brucellosis caused by Brucella canis in 72 dogs. The dogs were classified into three groups (infected, non-infected and suspected brucellosis) according to the results of blood culture and serological tests. The sensitivities of blood PCR and serum PCR were, respectively, 97.14 per cent and 25.71 per cent. The specificities of both were 100 per cent. In the group of dogs with suspected brucellosis, three were positive by blood PCR and none was positive by serum PCR. Serum PCR showed little value for the direct diagnosis of canine brucellosis as the assay had low diagnostic sensitivity and fewer positive dogs were detected by this test than by blood culture, blood PCR, rapid slide agglutination test (RSAT) and RSAT with 2-mercaptoethanol.
Authors:
L B Keid; R M Soares; S A Vasconcellos; V R Salgado; J Megid; L J Richtzenhain
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Veterinary record     Volume:  167     ISSN:  2042-7670     ISO Abbreviation:  Vet. Rec.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-20     Completed Date:  2010-08-17     Revised Date:  2012-05-10    
Medline Journal Info:
Nlm Unique ID:  0031164     Medline TA:  Vet Rec     Country:  England    
Other Details:
Languages:  eng     Pagination:  96-9     Citation Subset:  IM    
Affiliation:
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Rua Prof Dr Orlando Marques de Paiva, 87, 05508-900, São Paulo, SP, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Agglutination Tests / methods,  veterinary
Animals
Brucella canis / genetics,  isolation & purification*
Brucellosis / blood,  diagnosis,  veterinary*
Dog Diseases / blood,  diagnosis*
Dogs
Female
Male
Polymerase Chain Reaction / methods,  veterinary*
Sensitivity and Specificity
Serum / microbiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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