Document Detail


Comparison of Open-Flow Microperfusion and Microdialysis Methodologies When Sampling Topically Applied Fentanyl and Benzoic Acid in Human Dermis Ex Vivo.
MedLine Citation:
PMID:  22419258     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
PURPOSE: To compare two sampling methods-dermal Open-Flow Microperfusion (dOFM) and dermal Microdialysis (dMD)-in an international joint experiment in a single-laboratory setting. Using human ex-vivo skin and topically administered Fentanyl and Benzoic Acid, and to guide to researchers in choosing the most efficient method for a given penetrant and give suggestions concerning critical choices for successful dermal sampling. METHODS: The dOFM and dMD techniques are compared in equal set-ups using three probe-types (one dOFM probe and two dMD probe-types) in donor skin (n = 9) - 27 probes of each type sampling each penetrant in solutions applied in penetrationchambers glued to the skin surface over a time range of 20 h. RESULTS: Pharmacokinetic results demonstrated concordance between dOFM and dMD sampling technique under the given experimental conditions. The methods each had advantages and limitations in technical, practical and hands-on comparisons. CONCLUSION: When planning a study of cutaneous penetration the advantages and limitations of each probe-type have to be considered in relation to the scientific question posed, the physico-chemical characteristics of the substance of interest, the choice of experimental setting e.g. ex vivo/in vivo and the analytical skills available.
Authors:
R Holmgaard; E Benfeldt; J B Nielsen; C Gatschelhofer; J A Sorensen; C Höfferer; M Bodenlenz; T R Pieber; F Sinner
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-15
Journal Detail:
Title:  Pharmaceutical research     Volume:  -     ISSN:  1573-904X     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406521     Medline TA:  Pharm Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Environmental Medicine, University of Southern Denmark, Odense, Denmark, rikkeholmgaard@gmail.com.
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