Document Detail

A Comparison of Oleic Acid Metabolism in the Soybean (Glycine max [L.] Merr.) Genotypes Williams and A5, a Mutant with Decreased Linoleic Acid in the Seed.
MedLine Citation:
PMID:  16664804     Owner:  NLM     Status:  PubMed-not-MEDLINE    
The metabolism of oleoyl coenzyme A (CoA) was examined in developing seed from two soybean (Glycine max [L.] Merr.) genotypes: Williams, a standard cultivar and A5, a mutant containing nearly twice the oleic acid (18:1) content of Williams. The in vitro rates of esterification of oleoyl-CoA to lysophosphatides by acyl-CoA: lysophosphatidylcholine acyltransferase was similar in both genotypes and lysophosphatidyl-ethanolamine was a poor substrate. Crude extracts desaturated exogenous [1-(14)C]dioleoyl phosphatidylcholine at 14% of the rate achieved with [1-(14)C]oleoyl-CoA, and 50 micromolar lysophosphatidylcholine. The desaturase enzyme also required NADH for full activity. Extracts from Williams contained 1.5-fold more oleoyl phosphatidylcholine desaturase activity, on a fresh weight basis, than did A5 and appeared to have a similar affinity for oleoyl-CoA. There was 1.2- to 1.9-fold more linoleic acid (18:2) in phosphatidylcholine from Williams than from A5, measured at two stages of development, but both genotypes had a similar distribution of fatty acids in the one and two positions. Phosphatidylethanolamine in A5 contained relatively more linoleic acid (18:2) in the one position than did Williams. The increased oleic acid (18:1) content in A5 appeared to be a result of decreased rates of 18:1 desaturation of oleoyl-phosphatidylcholine in this genotype.
B A Martin; R W Rinne
Related Documents :
10336454 - Crystal structure of the mycobacterium tuberculosis enoyl-acp reductase, inha, in compl...
11171164 - Metabolic control analysis of de novo sunflower fatty acid biosynthesis.
6489524 - Malonyl coa inhibition of carnitine palmityltransferase in rat heart mitochondria.
10085244 - High-affinity binding of very-long-chain fatty acyl-coa esters to the peroxisomal non-s...
8282364 - Renal response to amino acid infusion in essential hypertension.
2210504 - Effect of juvenile hormone on musca domestica vicina macq. ii. the gonotrophic cycle.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  81     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1986 May 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  41-4     Citation Subset:  -    
Pioneer Hi-Bred International, Inc., Plant Breeding Division, Department of Research Specialists, P.O. Box 85, Johnston, Iowa 50131-0085.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Amino Acid Transport in Suspension-Cultured Plant Cells : VI. Influence of pH Buffers, Calcium, and ...
Next Document:  Continuous Monitoring of Plant Water Potential.