| A Comparison of Oleic Acid Metabolism in the Soybean (Glycine max [L.] Merr.) Genotypes Williams and A5, a Mutant with Decreased Linoleic Acid in the Seed. | |
| | |
MedLine Citation:
|
PMID: 16664804 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
|
The metabolism of oleoyl coenzyme A (CoA) was examined in developing seed from two soybean (Glycine max [L.] Merr.) genotypes: Williams, a standard cultivar and A5, a mutant containing nearly twice the oleic acid (18:1) content of Williams. The in vitro rates of esterification of oleoyl-CoA to lysophosphatides by acyl-CoA: lysophosphatidylcholine acyltransferase was similar in both genotypes and lysophosphatidyl-ethanolamine was a poor substrate. Crude extracts desaturated exogenous [1-(14)C]dioleoyl phosphatidylcholine at 14% of the rate achieved with [1-(14)C]oleoyl-CoA, and 50 micromolar lysophosphatidylcholine. The desaturase enzyme also required NADH for full activity. Extracts from Williams contained 1.5-fold more oleoyl phosphatidylcholine desaturase activity, on a fresh weight basis, than did A5 and appeared to have a similar affinity for oleoyl-CoA. There was 1.2- to 1.9-fold more linoleic acid (18:2) in phosphatidylcholine from Williams than from A5, measured at two stages of development, but both genotypes had a similar distribution of fatty acids in the one and two positions. Phosphatidylethanolamine in A5 contained relatively more linoleic acid (18:2) in the one position than did Williams. The increased oleic acid (18:1) content in A5 appeared to be a result of decreased rates of 18:1 desaturation of oleoyl-phosphatidylcholine in this genotype. |
| | |
Authors:
|
B A Martin; R W Rinne |
Related Documents
:
|
6552184 - Medium-chain fatty acid synthesis by goat mammary-gland fatty acid synthetase. the effe... 4966574 - De novo synthesis and elongation of fatty acids by subcellar fractions of monkey aorta. 15898114 - Lipid analysis of the sex pheromone gland of the moth heliothis virescens. 7556184 - Localization of the palmitoylation site in the transmembrane protein p12e of friend mur... 10698964 - Effects of fatty acids on uncoupling protein-2 expression in the rat heart. 14641014 - Regulation of pyruvate dehydrogenase complex activity by reversible phosphorylation. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Plant physiology Volume: 81 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 1986 May |
Date Detail:
|
Created Date: 2010-06-29 Completed Date: 2010-06-29 Revised Date: 2010-09-14 |
Medline Journal Info:
|
Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States |
Other Details:
|
Languages: eng Pagination: 41-4 Citation Subset: - |
Affiliation:
|
Pioneer Hi-Bred International, Inc., Plant Breeding Division, Department of Research Specialists, P.O. Box 85, Johnston, Iowa 50131-0085. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Amino Acid Transport in Suspension-Cultured Plant Cells : VI. Influence of pH Buffers, Calcium, and ...
Next Document: Continuous Monitoring of Plant Water Potential.